|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IL-1F6 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||HG10607-ACG|
|Human IL-1F6 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||HG10607-ACR|
|Human IL-1F6 Gene ORF cDNA clone expression plasmid, C-Flag tag||HG10607-CF|
|Human IL-1F6 Gene ORF cDNA clone expression plasmid, C-His tag||HG10607-CH|
|Human IL-1F6 Gene ORF cDNA clone expression plasmid, C-Myc tag||HG10607-CM|
|Human IL-1F6 Gene ORF cDNA clone expression plasmid, C-HA tag||HG10607-CY|
|Human IL-1F6 Gene ORF cDNA clone in cloning vector||HG10607-M|
|Human IL-1F6 Gene ORF cDNA clone expression plasmid, N-Flag tag||HG10607-NF|
|Human IL-1F6 Gene ORF cDNA clone expression plasmid, N-His tag||HG10607-NH|
|Human IL-1F6 Gene ORF cDNA clone expression plasmid, N-Myc tag||HG10607-NM|
|Human IL-1F6 Gene ORF cDNA clone expression plasmid, N-HA tag||HG10607-NY|
|Human IL-1F6 Gene ORF cDNA clone expression plasmid||HG10607-UT|
|Learn more about expression Vectors|
Interleukin-1 family member 6 (IL-1F6), also known as interleukin 36, alpha (IL36A), is a pro-inflammatory cytokine which plays an important role in innate and adaptive immunity. IL-1F6 activates MAPK and NF-kB pathways and is produced by many different cells. This cytokine is a family member of interleukin-1 (IL-1) and plays an important role in the pathophysiology of several diseases. It has been reported that IL-1F6 and IL-1F8, in addition to IL-1F9, activate the pathway leading to NF-kappaB in an IL-1Rrp2-dependent manner in Jurkat cells as well as in multiple other human and mouse cell lines. Activation of the pathway leading to NF-kappaB by IL-1F6 and IL-1F8 follows a similar time course to activation by IL-1beta, suggesting that signaling by the novel family members occurs through a direct mechanism. In a mammary epithelial cell line, NCI/ADR-RES, which naturally expresses IL-1Rrp2, all three cytokines signal without further receptor transfection. IL-1Rrp2 antibodies block activation of the pathway leading to NF-kappaB by IL-1F6, IL-1F8, and IL-1F9 in both Jurkat and NCI/ADR-RES cells. Thus IL-1F6, IL-1F8, and IL-1F9 signal through IL-1Rrp2 and IL-1RAcP.