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Human IL18BP / IL18BPa ELISA Pair Set

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    Human IL18BP Materials provided
    Capture Ab:1 mg/mL of mouse anti-Human IL18BP / IL18BPa monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10357-MM02)
    Detection Ab:0.2 mg/mL of rabbit anti-Human IL18BP / IL18BPa polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
    Standard:Each vial contains 47 ng of recombinant Human IL18BP / IL18BPa. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/mL is recommended.
    Human IL18BP Sensitivity
    The minimum detectable dose of Human IL18BP / IL18BPa was determined to be approximately 46.88 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
    Human IL18BP Principle of the product
    The Human IL18BP / IL18BPa ELISA Pair Set is for the quantitative determination of Human IL18BP / IL18BPa.
    The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human IL18BP / IL18BPa coated on a 96-well plate. Standards and samples are added to the wells, and any Human IL18BP / IL18BPa present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human IL18BP / IL18BPa polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human IL18BP / IL18BPa present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
    Human IL18BP Storage
    IL18BP / IL18BPa Background

    Interleukin-18-binding protein (IL-18BP) is a constitutively expressed and secreted protein. IL-18BP is a cytokine receptor that belongs to the interleukin 1 receptor family. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. The adjacently located family members IL18 Receptor 1 (IL18R1) and IL18 receptor accessory protein (IL18RAP) may also be important in the development of asthma and atopy. IL-18 binding protein (IL-18BP) was only moderately elevated, resulting in a high level of biologically active free IL-18 in HPS. A severe IL-18/IL-18BP imbalance results in Th-1 lymphocyte and macrophage activation, which escapes control by NK-cell cytotoxicity and may allow for secondary HPS in patients with underlying diseases.

    Human IL18BP / IL18BPa References
  • Novick D, et al.. (2001) A novel IL-18BP ELISA shows elevated serum IL-18BP in sepsis and extensive decrease of free IL-18. Cytokine. 14(6): 334-42.
  • Mazodier K, et al.. (2005) Severe imbalance of IL-18/IL-18BP in patients with secondary hemophagocytic syndrome. Blood. 106(10): 3483-9.
  • Akira S. (2000) The role of IL-18 in innate immunity. Curr Opin Immunol. 12(1): 59-63.
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    Catalog: SEKA10357-15
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