Quick Order

Human IL-12 (IL12A & IL12B Heterodimer) ELISA Pair Set

DatasheetReviewsRelated ProductsProtocols
Human IL12A & IL12B Materials provided
Capture Ab:0.5 mg/mL of rabbit anti-IL12A-HIS+IL12B-HIS monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating.
Detection Ab:0.2 mg/mL rabbit anti-IL12A-HIS+IL12B-HIS monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 30 ng of recombinant IL12A-HIS+IL12B-HIS. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1.5 ng/mL is recommended.
Human IL12A & IL12B Sensitivity
The minimum detectable dose of Human IL-12 (IL12A & IL12B Heterodimer) was determined to be approximately 23.4375 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human IL12A & IL12B Principle of the product
The Human IL-12 (IL12A & IL12B Heterodimer) ELISA Pair Set is for the quantitative determination of Human IL-12 (IL12A & IL12B Heterodimer).
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL-12 (IL12A & IL12B Heterodimer) coated on a 96-well plate. Standards and samples are added to the wells, and any IL-12 (IL12A & IL12B Heterodimer) present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-IL-12 (IL12A & IL12B Heterodimer) monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of IL-12 (IL12A & IL12B Heterodimer) present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Human IL12A & IL12B Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Human IL12A & IL12B ELISA Pair Set Standard Curve
Product nameProduct name
Size / Price
Catalog: SEKCT011-5
List Price: 
Price:      (You Save: )
Size:
5 Plates
15 Plates
Quantity:+-
AvailabilityIn Stock
Bulk Discount InquiryAdd to Cart
Contact Us
Images
  • Human IL-12 (IL12A & IL12B Heterodimer) ELISA Pair Set
    Recently Viewed Items
      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"