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Human IL-12 (IL12A & IL12B Heterodimer) ELISA Pair Set

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Human IL12A & IL12B Materials provided
Capture Ab:0.5 mg/mL of rabbit anti-IL12A-HIS+IL12B-HIS monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating.
Detection Ab:0.2 mg/mL rabbit anti-IL12A-HIS+IL12B-HIS monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
Standard:Each vial contains 30 ng of recombinant IL12A-HIS+IL12B-HIS. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1.5 ng/mL is recommended.
Human IL12A & IL12B Sensitivity
The minimum detectable dose of Human IL-12 (IL12A & IL12B Heterodimer) was determined to be approximately 23.4375 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Human IL12A & IL12B Principle of the product
The Human IL-12 (IL12A & IL12B Heterodimer) ELISA Pair Set is for the quantitative determination of Human IL-12 (IL12A & IL12B Heterodimer).
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL-12 (IL12A & IL12B Heterodimer) coated on a 96-well plate. Standards and samples are added to the wells, and any IL-12 (IL12A & IL12B Heterodimer) present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-IL-12 (IL12A & IL12B Heterodimer) monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of IL-12 (IL12A & IL12B Heterodimer) present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
Human IL12A & IL12B Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized Standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Human IL12A & IL12B ELISA Pair Set Standard Curve
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Catalog: SEKCT011-5
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