|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IGFBP4 Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||HG10967-ACG|
|Human IGFBP4 Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||HG10967-ACR|
|Human IGFBP4 Gene ORF cDNA clone expression plasmid, C-Flag tag||HG10967-CF|
|Human IGFBP4 Gene ORF cDNA clone expression plasmid, C-His tag||HG10967-CH|
|Human IGFBP4 Gene ORF cDNA clone expression plasmid, C-Myc tag||HG10967-CM|
|Human IGFBP4 Gene ORF cDNA clone expression plasmid, C-HA tag||HG10967-CY|
|Human IGFBP4 Gene ORF cDNA clone in cloning vector||HG10967-M|
|Human IGFBP4 Gene ORF cDNA clone expression plasmid, N-Flag tag||HG10967-NF|
|Human IGFBP4 Gene ORF cDNA clone expression plasmid, N-His tag||HG10967-NH|
|Human IGFBP4 Gene ORF cDNA clone expression plasmid, N-Myc tag||HG10967-NM|
|Human IGFBP4 Gene ORF cDNA clone expression plasmid, N-HA tag||HG10967-NY|
|Human IGFBP4 Gene ORF cDNA clone expression plasmid||HG10967-UT|
|Learn more about expression Vectors|
Insulin-like growth factor binding protein 4 (IGFBP-4) is a 24-kDa protein that binds insulin-like growth factor 1 (IGF-1) and IGF-2 with high affinity and inhibits IGF action in vitro. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are clearly distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF, or act in an IGF-independent manner in the prostate. IGF-binding protein-4 (IGFBP-4) inhibits IGF-I action in vitro and is the most abundant IGFBP in the rodent arterial wall. Expression of IGFBP-4 mRNA in nontransgenic littermates was maximal in liver and kidney. IGFBP-4 is a functional antagonist of IGF-I action on SMC. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence and IGF affinity.