|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human IFNG ORF mammalian expression plasmid, C-GFPSpark tag||HG11725-ACG|
|Human IFNG ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11725-ACR|
|Human IFNG ORF mammalian expression plasmid, C-Flag tag||HG11725-CF|
|Human IFNG ORF mammalian expression plasmid, C-His tag||HG11725-CH|
|Human IFNG ORF mammalian expression plasmid, C-Myc tag||HG11725-CM|
|Human IFNG ORF mammalian expression plasmid, C-HA tag||HG11725-CY|
|Human IFNG Gene cDNA clone plasmid||HG11725-G|
|Human IFNG ORF mammalian expression plasmid, N-Flag tag||HG11725-NF|
|Human IFNG ORF mammalian expression plasmid, N-His tag||HG11725-NH|
|Human IFNG ORF mammalian expression plasmid, N-Myc tag||HG11725-NM|
|Human IFNG ORF mammalian expression plasmid, N-HA tag||HG11725-NY|
|Human IFNG natural ORF mammalian expression plasmid||HG11725-UT|
|Learn more about expression Vectors|
IFN gamma, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.