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Human Insulysin/insulin-degrading enzyme Gene ORF cDNA clone expression plasmid, N-His tag

  • Human Insulysin/insulin-degrading enzyme Gene Plasmid Map 15908
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Human IDE cDNA Clone Product Information
NCBI RefSeq:BC096336
RefSeq ORF Size:3105 bp
cDNA Description:Full length Clone DNA of Homo sapiens insulin-degrading enzyme with N terminal His tag.
Gene Synonym:INSULYSIN
Species:Human
Vector:pCMV3-N-His
Plasmid:pCMV3-His-IDE
Restriction Site:KpnI(two restriction sites) + XbaI(6kb+3.06kb+0.06kb)
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with IDE qPCR primers for gene expression analysis, HP104565 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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