HGF qPCR Primer Pairs, Human

Cat: HP100491
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HGF qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
HGF qPCR Primer Pairs, Human Alternative Names
DFNB39 qPCR Primer Pairs, Human;F-TCF qPCR Primer Pairs, Human;F-TCFB qPCR Primer Pairs, Human;Hepatocyte Growth Factor qPCR Primer Pairs, Human;HGFB qPCR Primer Pairs, Human;HPTA qPCR Primer Pairs, Human;SF qPCR Primer Pairs, Human
HGF Background Information

Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. Hepatocyte growth factor is secreted by mesenchymal cellsas a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
hepatocyte growth factor (hepapoietin A; scatter factor)
References
  • Naldini L, et al. (1991) Scatter factor and hepatocyte growth factor are indistinguishable ligands for the MET receptor. EMBO J. 10(10):2867-78.
  • Comoglio, et al. (1993) Structure, biosynthesis and biochemical properties of the HGF receptor in normal and malignant cells. 65:131-65.
  • Hahn W, et al. (2011) Enhanced cardioprotective effects by coexpression of two isoforms of hepatocyte growth factor from naked plasmid DNA in a rat ischemic heart disease model. The Journal of Gene Medicine. 13(10):549-55.
  • Bottaro DP, et al. (1991) Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product. Science. 251(4995):802-4.
  • HGF qPCR Primer Pairs, Rhesus, Related Products

    Product Name Catalog#(PDF)
    HGF cDNA ORF Clone in Cloning Vector, Rhesus CG90286-G
    HGF cDNA ORF Clone, Rhesus, untagged CG90286-UT
    HGF Lentiviral cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90286-ACGLN
    HGF cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90286-ACG
    HGF cDNA ORF Clone, Rhesus, C-OFPSpark® tag CG90286-ACR
    HGF cDNA ORF Clone, Rhesus, C-DDK (Flag®) tag CG90286-CF
    HGF cDNA ORF Clone, Rhesus, N-DDK (Flag®) tag CG90286-NF
    HGF cDNA ORF Clone, Rhesus, C-His tag CG90286-CH
    HGF cDNA ORF Clone, Rhesus, N-His tag CG90286-NH
    HGF cDNA ORF Clone, Rhesus, C-HA tag CG90286-CY
    HGF cDNA ORF Clone, Rhesus, N-HA tag CG90286-NY
    HGF cDNA ORF Clone, Rhesus, C-Myc tag CG90286-CM
    HGF cDNA ORF Clone, Rhesus, N-Myc tag CG90286-NM

    HGF qPCR Primer Pairs, Canine, Related Products

    Product Name Catalog#(PDF)
    HGF cDNA ORF Clone in Cloning Vector, Canine DG70063-G
    HGF cDNA ORF Clone, Canine, untagged DG70063-UT
    HGF Lentiviral cDNA ORF Clone, Canine, C-GFPSpark® tag DG70063-ACGLN
    HGF cDNA ORF Clone, Canine, C-GFPSpark® tag DG70063-ACG
    HGF cDNA ORF Clone, Canine, C-OFPSpark® tag DG70063-ACR
    HGF cDNA ORF Clone, Canine, C-DDK (Flag®) tag DG70063-CF
    HGF cDNA ORF Clone, Canine, N-DDK (Flag®) tag DG70063-NF
    HGF cDNA ORF Clone, Canine, C-His tag DG70063-CH
    HGF cDNA ORF Clone, Canine, N-His tag DG70063-NH
    HGF cDNA ORF Clone, Canine, C-HA tag DG70063-CY
    HGF cDNA ORF Clone, Canine, N-HA tag DG70063-NY
    HGF cDNA ORF Clone, Canine, C-Myc tag DG70063-CM
    HGF cDNA ORF Clone, Canine, N-Myc tag DG70063-NM

    HGF qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    HGF cDNA ORF Clone in Cloning Vector, Rat RG80429-G
    HGF cDNA ORF Clone, Rat, untagged RG80429-UT
    HGF cDNA ORF Clone, Rat, C-GFPSpark® tag RG80429-ACG
    HGF Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80429-ACGLN
    HGF cDNA ORF Clone, Rat, C-OFPSpark® tag RG80429-ACR
    HGF cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80429-CF
    HGF cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80429-NF
    HGF cDNA ORF Clone, Rat, C-His tag RG80429-CH
    HGF cDNA ORF Clone, Rat, N-His tag RG80429-NH
    HGF cDNA ORF Clone, Rat, C-HA tag RG80429-CY
    HGF cDNA ORF Clone, Rat, N-HA tag RG80429-NY
    HGF cDNA ORF Clone, Rat, C-Myc tag RG80429-CM
    HGF cDNA ORF Clone, Rat, N-Myc tag RG80429-NM

    HGF qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    HGF cDNA ORF Clone in Cloning Vector, Mouse MG50038-M
    HGF cDNA ORF Clone, Mouse, untagged MG50038-UT
    HGF cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50038-ACG
    HGF Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50038-ACGLN
    HGF cDNA ORF Clone, Mouse, N-GFPSpark® tag MG50038-ANG
    HGF cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50038-ACR
    HGF cDNA ORF Clone, Mouse, N-OFPSpark® tag MG50038-ANR
    HGF cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50038-CF
    HGF cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50038-NF
    HGF cDNA ORF Clone, Mouse, C-His tag MG50038-CH
    HGF cDNA ORF Clone, Mouse, N-His tag MG50038-NH
    HGF cDNA ORF Clone, Mouse, C-HA tag MG50038-CY
    HGF cDNA ORF Clone, Mouse, N-HA tag MG50038-NY
    HGF cDNA ORF Clone, Mouse, C-Myc tag MG50038-CM
    HGF cDNA ORF Clone, Mouse, N-Myc tag MG50038-NM

    HGF qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    HGF cDNA ORF Clone in Cloning Vector, Human HG10463-M
    HGF cDNA ORF Clone, Human, untagged HG10463-UT
    HGF cDNA ORF Clone, Human, C-GFPSpark® tag HG10463-ACG
    HGF Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10463-ACGLN
    HGF cDNA ORF Clone, Human, C-OFPSpark® tag HG10463-ACR
    HGF cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10463-CF
    HGF cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10463-NF
    HGF cDNA ORF Clone, Human, C-His tag HG10463-CH
    HGF cDNA ORF Clone, Human, N-His tag HG10463-NH
    HGF cDNA ORF Clone, Human, C-HA tag HG10463-CY
    HGF cDNA ORF Clone, Human, N-HA tag HG10463-NY
    HGF cDNA ORF Clone, Human, C-Myc tag HG10463-CM
    HGF cDNA ORF Clone, Human, N-Myc tag HG10463-NM
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