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Human HEXB / Hexosaminidase B Gene ORF cDNA clone expression plasmid, N-His tag

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Human HEXB cDNA Clone Product Information
NCBI RefSeq:NM_000521.3
RefSeq ORF Size:1671bp
cDNA Description:Full length Clone DNA of Homo sapiens hexosaminidase B (beta polypeptide) with N terminal His tag.
Gene Synonym:ENC-1AS
Species:Human
Vector:pCMV3-SP-N-His
Plasmid:pCMV3-His-HEXB
Restriction Site:KpnI + XbaI (6kb + 1.64kb)
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Human HEXB Gene Plasmid Map
Human HEXB ORF mammalian expression plasmid, N-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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Catalog: HG13169-NH
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  • Human HEXB ORF mammalian expression plasmid, N-His tag
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