DAP10 cDNA ORF Clone, Human, C-HA tag General Information
Gene
Description
Full length Clone DNA of Human hematopoietic cell signal transducer with C terminal HA tag.
Plasmid
Promoter
Enhanced CMV promoter
Tag Sequence
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
DAP10 cDNA ORF Clone, Human, C-HA tag Alternative Names
DAP10 cDNA ORF Clone, Human;KAP10 cDNA ORF Clone, Human;PIK3AP cDNA ORF Clone, Human
DAP10 Background Information
HCST, also known as DAP10, belongs to the DAP10 family. It is a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. HCST may be part of the immune recognition receptor complex. This receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, and UL16-binding proteins (ULBPs). It may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif.
Full Name
hematopoietic cell signal transducer
References
Wu J. et al., 1999, Science. 285 (5428): 730-2.Karimi M. et al., 2006, J Immunol. 175 (12): 7819-28.André P. et al., 2004, Eur J Immunol. 34 (4): 961-71.
