|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human GNB2L1 ORF mammalian expression plasmid, C-GFPSpark tag||HG16196-ACG|
|Human GNB2L1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG16196-ACR|
|Human GNB2L1 ORF mammalian expression plasmid, C-Flag tag||HG16196-CF|
|Human GNB2L1 ORF mammalian expression plasmid, C-His tag||HG16196-CH|
|Human GNB2L1 ORF mammalian expression plasmid, C-Myc tag||HG16196-CM|
|Human GNB2L1 ORF mammalian expression plasmid, C-HA tag||HG16196-CY|
|Human GNB2L1 Gene cDNA clone plasmid||HG16196-G|
|Human GNB2L1 ORF mammalian expression plasmid, N-Flag tag||HG16196-NF|
|Human GNB2L1 ORF mammalian expression plasmid, N-His tag||HG16196-NH|
|Human GNB2L1 ORF mammalian expression plasmid, N-Myc tag||HG16196-NM|
|Human GNB2L1 ORF mammalian expression plasmid, N-HA tag||HG16196-NY|
|Human GNB2L1 natural ORF mammalian expression plasmid||HG16196-UT|
|Learn more about expression Vectors|
RACK1 (guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1), also known as GNB2L1, contains 7 WD repeats and belongs to the WD repeat G protein beta family. In the liver, RACK1 is expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. It is up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue. RACK1 is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. It interacts with a wide variety of proteins and plays a role in many cellular processes. GNB2L1 binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. RACK1 may recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. It inhibits the activity of SRC kinases including SRC, LCK and YES1. RACK1 also inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. It enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer.