|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Human GIF ORF mammalian expression plasmid, C-GFPSpark tag||HG13544-ACG|
|Human GIF ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13544-ACR|
|Human GIF ORF mammalian expression plasmid, C-Flag tag||HG13544-CF|
|Human GIF ORF mammalian expression plasmid, C-His tag||HG13544-CH|
|Human GIF ORF mammalian expression plasmid, C-Myc tag||HG13544-CM|
|Human GIF ORF mammalian expression plasmid, C-HA tag||HG13544-CY|
|Human GIF Gene cDNA clone plasmid||HG13544-G|
|Human GIF ORF mammalian expression plasmid, N-Flag tag||HG13544-NF|
|Human GIF ORF mammalian expression plasmid, N-His tag||HG13544-NH|
|Human GIF ORF mammalian expression plasmid, N-Myc tag||HG13544-NM|
|Human GIF ORF mammalian expression plasmid, N-HA tag||HG13544-NY|
|Human GIF natural ORF mammalian expression plasmid||HG13544-UT|
|Learn more about expression Vectors|
Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver.