|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FUT8 ORF mammalian expression plasmid, C-GFPSpark tag||HG11326-ACG|
|Human FUT8 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11326-ACR|
|Human FUT8 ORF mammalian expression plasmid, C-Flag tag||HG11326-CF|
|Human FUT8 ORF mammalian expression plasmid, C-His tag||HG11326-CH|
|Human FUT8 ORF mammalian expression plasmid, C-Myc tag||HG11326-CM|
|Human FUT8 ORF mammalian expression plasmid, C-HA tag||HG11326-CY|
|Human FUT8 Gene cDNA clone plasmid||HG11326-M|
|Human FUT8 ORF mammalian expression plasmid, N-Flag tag||HG11326-NF|
|Human FUT8 ORF mammalian expression plasmid, N-His tag||HG11326-NH|
|Human FUT8 ORF mammalian expression plasmid, N-Myc tag||HG11326-NM|
|Human FUT8 ORF mammalian expression plasmid, N-HA tag||HG11326-NY|
|Human FUT8 natural ORF mammalian expression plasmid||HG11326-UT|
|Learn more about expression Vectors|
Human alpha (1,6) fucosyltransferase 8, also known as FUT8, is a member of the glycosyltransferase family. Fucosyltransferases are the enzymes transferring fucose from GDP-Fuc to Gal in an alpha1,2-linkage and to GlcNAc in alpha1,3-linkage, alpha1,4-linkage, or alpha1,6-linkage. All fucosyltransferases utilize the same nucleotide sugar, their specificity reside in the recognition of the acceptor and in the type of linkage formed. Fucosyltransferases share some common structural and catalytic features. On the basis of protein sequence similarities, these enzymes can be classified into four distinct families: (1) the alpha-2-fucosyltransferases, (2) the alpha-3-fucosyltransferases, (3) the mammalian alpha-6-fucosyltransferases, and (4) the bacterial alpha-6-fucosyltransferases. The alpha-3-fucosyltransferases constitute a distinct family as they lack the consensus peptide, but some regions display similarities with the alpha-2 and alpha-6-fucosyltranferases.