Human FSH Beta HEK293 Overexpression Lysate

Cat: 11935-H08HL
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Human FSH Beta HEK293 Overexpression Lysate Product Information
Product Description
This Human FSH Beta overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of FSH Beta protein (Cat: 11935-H08H) from the overexpression lysate was verified.
Expression Host
HEK293 Cells
Species
Human
Sequence Information
A DNA sequence encoding the human FSHB (NP_000501.1) (Met1-Glu129) was expressed with a polyhistidine tag at the C-terminus.
Molecule Mass
The recombinant human FSHB consists 122 amino acids and predicts a molecular mass of 13.9 kDa.
Human FSH Beta HEK293 Overexpression Lysate Usage Guide
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Recommend Usage
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
Sample Buffer
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Stability & Storage
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Application
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.
Human FSH Beta HEK293 Overexpression Lysate Alternative Names
Human HH24 Overexpression Lysate
FSH Beta Background Information
Full Name
follicle stimulating hormone, beta polypeptide

Human Overexpression Lysate

Product Name Cat.No.
Human FSH Beta HEK293 Overexpression Lysate 11935-H02HL
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