|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FLRT2 ORF mammalian expression plasmid, C-GFPSpark tag||HG11296-ACG|
|Human FLRT2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG11296-ACR|
|Human FLRT2 ORF mammalian expression plasmid, C-Flag tag||HG11296-CF|
|Human FLRT2 ORF mammalian expression plasmid, C-His tag||HG11296-CH|
|Human FLRT2 ORF mammalian expression plasmid, C-Myc tag||HG11296-CM|
|Human FLRT2 ORF mammalian expression plasmid, C-HA tag||HG11296-CY|
|Human FLRT2 Gene cDNA clone plasmid||HG11296-M|
|Human FLRT2 ORF mammalian expression plasmid, N-Flag tag||HG11296-NF|
|Human FLRT2 ORF mammalian expression plasmid, N-His tag||HG11296-NH|
|Human FLRT2 ORF mammalian expression plasmid, N-Myc tag||HG11296-NM|
|Human FLRT2 ORF mammalian expression plasmid, N-HA tag||HG11296-NY|
|Human FLRT2 natural ORF mammalian expression plasmid||HG11296-UT|
|Learn more about expression Vectors|
Fibronectin Leucine-Rich Transmembrane (FLRT) proteins are glycosylated membrane proteins expressed at the cell surface which localise in a homophilic manner to cell-cell contacts expressing the focal adhesion marker vinculin. FLRT1, FLRT2, and FLRT3, the three genes encode putative type I transmembrane proteins, each containing 10 leucine-rich repeats (LRR), a type III fibronectin (FN) domain, followed by the transmembrane region, and a short cytoplasmic tail. FLRT family members may function in cell adhesion and/or receptor signalling. Each member of the FLRT family has a distinct, highly regulated expression pattern, as was seen for the NLRR family. FLRT2 is expressed in a subset of the sclerotome, adjacent to the region that forms the syndetome, suggesting that interaction with FGF signalling may be a general property of FLRT proteins. All FLRTs can interact with FGFR1 and FLRTs can be induced by the activation of FGF signalling by FGF-2. FLRT proteins have a dual role, promoting FGF signalling and modulating homotypic cell adhesion. FLRT2 played critical roles in craniofacial development, and it was also present in the vomero-nasal organ, mandibular primodia, and the posterior aspects of the unfused and fused secondary palatal shelves.