|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human FKBP14 ORF mammalian expression plasmid, C-GFPSpark tag||HG13522-ACG|
|Human FKBP14 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG13522-ACR|
|Human FKBP14 ORF mammalian expression plasmid, C-Flag tag||HG13522-CF|
|Human FKBP14 ORF mammalian expression plasmid, C-His tag||HG13522-CH|
|Human FKBP14 ORF mammalian expression plasmid, C-Myc tag||HG13522-CM|
|Human FKBP14 ORF mammalian expression plasmid, C-HA tag||HG13522-CY|
|Human FKBP14 Gene cDNA clone plasmid||HG13522-G|
|Human FKBP14 ORF mammalian expression plasmid, N-Flag tag||HG13522-NF|
|Human FKBP14 ORF mammalian expression plasmid, N-His tag||HG13522-NH|
|Human FKBP14 ORF mammalian expression plasmid, N-Myc tag||HG13522-NM|
|Human FKBP14 ORF mammalian expression plasmid, N-HA tag||HG13522-NY|
|Human FKBP14 natural ORF mammalian expression plasmid||HG13522-UT|
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FKBP14 belongs to the FK506-binding protein family. It contains 2 EF-hand domains and one PPIase FKBP-type domain. FKBP14 can be detected in the lumen of the endoplasmic reticulum where it is thought to accelerate the folding of proteins during protein synthesis. Truncation of the amino-terminus of FKBP14 significantly decreases peptidyl prolyl cis-trans isomerase activity, therefore implicating that the PPIase FKBP-type domain must be located at the N-terminus. Defects in FKBP14 can cause Ehlers-Danlos syndrome with progressive kyphoscoliosis, myopathy, and hearing loss. A syndrome with features of Ehlers-Danlos syndrome types VIA and VIB on the one hand, and the collagen VI-related congenital myopathies Ullrich congenital muscular dystrophy and Bethlem myopathy on the other hand.