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Human CD32a/FCGR2A/Fc gamma RIIA transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag tag

  • Human FCGR2A / CD32a transcript variant 1 ORF mammalian expression plasmid, C-Flag tag
  • Human FCGR2A / CD32a transcript variant 1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-tagged
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Human FCGR2A cDNA Clone Product Information
NCBI RefSeq:NM_001136219.1
RefSeq ORF Size:954bp
cDNA Description:Full length Clone DNA of Homo sapiens Fc fragment of IgG, low affinity IIa, receptor (CD32), transcript variant 1 with C terminal Flag tag.
Gene Synonym:CD32, FCG2, FcGR, CD32A, CDw32, FCGR2, IGFR2, FCGR2A1, MGC23887, MGC30032
Species:Human
Vector:pCMV3-C-FLAG
Plasmid:pCMV3-FCGR2A-Flag
Restriction Site:KpnI + XbaI (6kb + 1kb)
Tag Sequence:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for the point mutations: 174G/T,600 T/C not causing the amino acid variation.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with FCGR2A qPCR primers for gene expression analysis, HP100409 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Human FCGR2A Gene Plasmid Map
Human FCGR2A / CD32a transcript variant 1 Gene cDNA Clone (full-length ORF Clone), expression ready, C-FLAG-tagged
Human FCGR2A Gene Expression validated Image
Human FCGR2A / CD32a transcript variant 1 ORF mammalian expression plasmid, C-Flag tag
[Click to enlarge image]
Caption:
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Human CD32a/FCGR2A/Fc gamma RIIA transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag tag on other vectors
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Datasheet & Documentation

All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"