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Human Estrogen Receptor alpha Gene ORF cDNA clone expression plasmid, N-HA tag

  • Human ESR1 ORF mammalian expression plasmid, N-HA tag
  • Human Estrogen Receptor alpha Gene Plasmid Map 5838
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Human ESR1 cDNA Clone Product Information
NCBI RefSeq:NM_001122742.1
RefSeq ORF Size:1788bp
cDNA Description:Full length Clone DNA of Homo sapiens estrogen receptor 1 with N terminal HA tag.
Gene Synonym:ER, ESR, Era, ESRA, NR3A1, DKFZp686N23123, ESR1
Species:Human
Vector:pCMV3-N-HA
Plasmid:pCMV3-HA-ESR1
Restriction Site:KpnI + XbaI(two restriction sites) (6kb + 1.18kb + 0.66kb)
Tag Sequence:HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with ESR1 qPCR primers for gene expression analysis, HP101765 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Human ESR1 Gene Plasmid Map
Human ESR1 ORF mammalian expression plasmid, N-HA tag
HA Tag Info

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.

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