EEF1E1 cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG14004-CF
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EEF1E1 cDNA ORF Clone, Human, C-DDK (Flag®) tag General Information
Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
525 bp
Description
Full length Clone DNA of Human eukaryotic translation elongation factor 1 epsilon 1 with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-FLAG
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

EEF1E1 cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
AIMP3 cDNA ORF Clone, Human;P18 cDNA ORF Clone, Human
EEF1E1 Background Information

EEF1E1, also known as AIMP3 and p18, is a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, EEF1E1 is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. It is comprised of a bifunctional glutamyl-prolyl-tRNA synthase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl and aspartyl-tRNA synthases, and three auxiliary proteins, EEF1E1/p18, AIMP2/p38 and AIMP1/p43. EEF1E1 also plays a positive role in ATM/ATR-mediated p53 activation.

Full Name
eukaryotic translation elongation factor 1 epsilon 1
References
  • Mao M. et al., 1998, Proc Natl Acad Sci. 95 (14): 8175-80.
  • Quevillon S. et al., 1999, J Mol Biol. 285 (1): 183-95.
  • Ahn HC. et al., 2003, FEBS Lett. 542 (1-3): 119-24.
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    EEF1E1 cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    EEF1E1 cDNA ORF Clone in Cloning Vector, Human HG14004-G
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    EEF1E1 cDNA ORF Clone, Human, N-HA tag HG14004-NY
    EEF1E1 cDNA ORF Clone, Human, C-Myc tag HG14004-CM
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