|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human EBAG9 ORF mammalian expression plasmid, C-GFPSpark tag||HG14012-ACG|
|Human EBAG9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14012-ACR|
|Human EBAG9 ORF mammalian expression plasmid, C-Flag tag||HG14012-CF|
|Human EBAG9 ORF mammalian expression plasmid, C-His tag||HG14012-CH|
|Human EBAG9 ORF mammalian expression plasmid, C-Myc tag||HG14012-CM|
|Human EBAG9 ORF mammalian expression plasmid, C-HA tag||HG14012-CY|
|Human EBAG9 Gene cDNA clone plasmid||HG14012-G|
|Human EBAG9 ORF mammalian expression plasmid, N-Flag tag||HG14012-NF|
|Human EBAG9 ORF mammalian expression plasmid, N-His tag||HG14012-NH|
|Human EBAG9 ORF mammalian expression plasmid, N-Myc tag||HG14012-NM|
|Human EBAG9 ORF mammalian expression plasmid, N-HA tag||HG14012-NY|
|Human EBAG9 natural ORF mammalian expression plasmid||HG14012-UT|
|Learn more about expression Vectors|
RCAS1, also known as EBAG9, is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. RCAS1 gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of RCAS1 gene. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for RCAS1 gene. EBAG9 may participate in suppression of cell proliferation and induces apoptotic cell death through activation of interleukin-1-beta converting enzyme (ICE)-like proteases.