Carboxypeptidase B1 qPCR Primer Pairs, Human

Cat: HP100522
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Carboxypeptidase B1 qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
Carboxypeptidase B1 qPCR Primer Pairs, Human Alternative Names
CPB qPCR Primer Pairs, Human;PASP qPCR Primer Pairs, Human;PCPB qPCR Primer Pairs, Human
Carboxypeptidase B1 Background Information

Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

Full Name
carboxypeptidase B1 (tissue)
References
  • Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581.
  • Pezzilli, R. et al., 1994, Digestion. 55: 73-77.
  • Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.
  • Carboxypeptidase B1 qPCR Primer Pairs, Rhesus, Related Products

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    Carboxypeptidase B1 cDNA ORF Clone, Rhesus, untagged CG90524-UT
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    Carboxypeptidase B1 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    Carboxypeptidase B1 cDNA ORF Clone in Cloning Vector, Mouse MG50386-M
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    Carboxypeptidase B1 cDNA ORF Clone, Mouse, C-His tag MG50386-CH
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    Carboxypeptidase B1 cDNA ORF Clone, Mouse, C-HA tag MG50386-CY
    Carboxypeptidase B1 cDNA ORF Clone, Mouse, N-HA tag MG50386-NY
    Carboxypeptidase B1 cDNA ORF Clone, Mouse, C-Myc tag MG50386-CM
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    Carboxypeptidase B1 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    Carboxypeptidase B1 cDNA ORF Clone in Cloning Vector, Human HG10501-M
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    Carboxypeptidase B1 cDNA ORF Clone, Human, C-GFPSpark® tag HG10501-ACG
    Carboxypeptidase B1 cDNA ORF Clone, Human, C-OFPSpark® tag HG10501-ACR
    Carboxypeptidase B1 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10501-CF
    Carboxypeptidase B1 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10501-NF
    Carboxypeptidase B1 cDNA ORF Clone, Human, C-His tag HG10501-CH
    Carboxypeptidase B1 cDNA ORF Clone, Human, N-His tag HG10501-NH
    Carboxypeptidase B1 cDNA ORF Clone, Human, C-HA tag HG10501-CY
    Carboxypeptidase B1 cDNA ORF Clone, Human, N-HA tag HG10501-NY
    Carboxypeptidase B1 cDNA ORF Clone, Human, C-Myc tag HG10501-CM
    Carboxypeptidase B1 cDNA ORF Clone, Human, N-Myc tag HG10501-NM
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