COCH qPCR Primer Pairs, Human

Cat: HP101250
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All COCH Reagents
CRO Services
Product recommended

All COCH Reagents
COCH qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
COCH qPCR Primer Pairs, Human Alternative Names
COCH-5B2 qPCR Primer Pairs, Human;COCH5B2 qPCR Primer Pairs, Human;DFNA9 qPCR Primer Pairs, Human
COCH Background Information

Cochlin, also known as COCH-5B2 and COCH, is a secreted protein which contains one LCCL domain and two VWFA domains. It is an abundant inner ear protein expressed as multiple isoforms. Its function is also unknown, but it is suspected to be an extracellular matrix component. Cochlin and type II collagen are major constituents of the inner ear extracellular matrix, and Cochlin constitutes 70% of non-collagenous protein in the inner ear, the cochlin isoforms can be classified into three subgroups, p63s, p44s and p40s. The expression of cochlin is highly specific to the inner ear. Eleven missense mutation and one in-frame deletion have been reported in the COCH gene, causing hereditary progressive sensorineural hearing loss and vestibular dysfunction, deafness autosomal dominant type 9 (DFNA9). The co-localization of cochlin and type II collagen in the fibrillar substance in the subepithelial area indicate that cochlin may play a role in the structural homeostasis of the vestibule acting in concert with the fibrillar type II collagen bundles. Defects in COCH may contribute to Meniere disease which is an autosomal dominant disorder characterized by hearing loss associated with episodic vertigo.

Full Name
cochlin
References
  • Ikezono T, et al. (2005) Expression of cochlin in the vestibular organ of rats. ORL J Otorhinolaryngol Relat Spec. 67(5): 252-8.
  • Shindo S, et al. (2008) Spatiotemporal expression of cochlin in the inner ear of rats during postnatal development. Neurosci Lett. 444(2): 148-52.
  • Hosokawa S, et al. (2010) Ultrastructural localization of cochlin in the rat cochlear duct. Audiol Neurootol. 15(4): 247-53.
  • COCH qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    COCH cDNA ORF Clone in Cloning Vector, Mouse MG50943-G
    COCH cDNA ORF Clone, Mouse, untagged MG50943-UT
    COCH Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50943-ACGLN
    COCH cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50943-ACG
    COCH cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50943-ACR
    COCH cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50943-CF
    COCH cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50943-NF
    COCH cDNA ORF Clone, Mouse, C-His tag MG50943-CH
    COCH cDNA ORF Clone, Mouse, N-His tag MG50943-NH
    COCH cDNA ORF Clone, Mouse, C-HA tag MG50943-CY
    COCH cDNA ORF Clone, Mouse, N-HA tag MG50943-NY
    COCH cDNA ORF Clone, Mouse, C-Myc tag MG50943-CM
    COCH cDNA ORF Clone, Mouse, N-Myc tag MG50943-NM

    COCH qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    COCH cDNA ORF Clone in Cloning Vector, Human HG11368-M
    COCH cDNA ORF Clone, Human, untagged HG11368-UT
    COCH cDNA ORF Clone, Human, C-GFPSpark® tag HG11368-ACG
    COCH Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11368-ACGLN
    COCH cDNA ORF Clone, Human, C-OFPSpark® tag HG11368-ACR
    COCH cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11368-CF
    COCH cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11368-NF
    COCH cDNA ORF Clone, Human, C-His tag HG11368-CH
    COCH cDNA ORF Clone, Human, N-His tag HG11368-NH
    COCH cDNA ORF Clone, Human, C-HA tag HG11368-CY
    COCH cDNA ORF Clone, Human, N-HA tag HG11368-NY
    COCH cDNA ORF Clone, Human, C-Myc tag HG11368-CM
    COCH cDNA ORF Clone, Human, N-Myc tag HG11368-NM
    info info
    添加购物车成功! 添加购物车失败!