UMP / CMP kinase qPCR Primer Pairs, Human

Cat: HP102908
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UMP / CMP kinase qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
UMP / CMP kinase qPCR Primer Pairs, Human Alternative Names
CK qPCR Primer Pairs, Human;CMK qPCR Primer Pairs, Human;CMPK qPCR Primer Pairs, Human;UMK qPCR Primer Pairs, Human;UMP-CMPK qPCR Primer Pairs, Human;UMPK qPCR Primer Pairs, Human
UMP / CMP kinase Background Information

CMPK1 plays a key role in the maintenance of pyrimidine nucleotide pool profile and for the metabolism of pyrimidine analogs in cells. It catalyzes the phosphoryl transfer from ATP to UMP, CMP, and deoxy-CMP (dCMP), resulting in the formation of ADP and the corresponding nucleoside diphosphate. CMPK1 also has a significant role in the activation of pyrimidine analogs, which are clinically useful anti-cancer and anti-viral drugs. In the meanwhile, CMPK1 functions in cellular nucleic acid biosynthesis.

Full Name
cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
References
  • Liou J, et al. (2004) Phosphorylation of Cytidine, Deoxycytidine, and Their Analog Monophosphates by Human UMP/CMP Kinase is Differentially Regulated by ATP and Magnesium. Molecular Pharmacology. 67(3):806-14.
  • Gerhard DS, et al. (2004) The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC). Genome Research. 14(10B):2121-7.
  • Segura-Pena D, et al. (2004) Substrate-induced Conformational Changes in Human UMP/CMP Kinase. Journal of Biological Chemistry. 279(32):33882-9.
  • UMP / CMP kinase qPCR Primer Pairs, Canine, Related Products

    Product Name Catalog#(PDF)
    UMP / CMP kinase cDNA ORF Clone in Cloning Vector, Canine DG70228-U
    UMP / CMP kinase cDNA ORF Clone, Canine, untagged DG70228-UT
    UMP / CMP kinase cDNA ORF Clone, Canine, C-GFPSpark® tag DG70228-ACG
    UMP / CMP kinase Lentiviral cDNA ORF Clone, Canine, C-GFPSpark® tag DG70228-ACGLN
    UMP / CMP kinase cDNA ORF Clone, Canine, N-GFPSpark® tag DG70228-ANG
    UMP / CMP kinase cDNA ORF Clone, Canine, C-OFPSpark® tag DG70228-ACR
    UMP / CMP kinase cDNA ORF Clone, Canine, N-OFPSpark® tag DG70228-ANR
    UMP / CMP kinase cDNA ORF Clone, Canine, C-DDK (Flag®) tag DG70228-CF
    UMP / CMP kinase cDNA ORF Clone, Canine, N-DDK (Flag®) tag DG70228-NF
    UMP / CMP kinase cDNA ORF Clone, Canine, C-His tag DG70228-CH
    UMP / CMP kinase cDNA ORF Clone, Canine, N-His tag DG70228-NH
    UMP / CMP kinase cDNA ORF Clone, Canine, C-HA tag DG70228-CY
    UMP / CMP kinase cDNA ORF Clone, Canine, N-HA tag DG70228-NY
    UMP / CMP kinase cDNA ORF Clone, Canine, C-Myc tag DG70228-CM
    UMP / CMP kinase cDNA ORF Clone, Canine, N-Myc tag DG70228-NM

    UMP / CMP kinase qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    UMP / CMP kinase cDNA ORF Clone in Cloning Vector, Rat RG81090-U
    UMP / CMP kinase cDNA ORF Clone, Rat, untagged RG81090-UT
    UMP / CMP kinase cDNA ORF Clone, Rat, C-GFPSpark® tag RG81090-ACG
    UMP / CMP kinase Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG81090-ACGLN
    UMP / CMP kinase cDNA ORF Clone, Rat, N-GFPSpark® tag RG81090-ANG
    UMP / CMP kinase cDNA ORF Clone, Rat, C-OFPSpark® tag RG81090-ACR
    UMP / CMP kinase cDNA ORF Clone, Rat, N-OFPSpark® tag RG81090-ANR
    UMP / CMP kinase cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG81090-CF
    UMP / CMP kinase cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG81090-NF
    UMP / CMP kinase cDNA ORF Clone, Rat, C-His tag RG81090-CH
    UMP / CMP kinase cDNA ORF Clone, Rat, N-His tag RG81090-NH
    UMP / CMP kinase cDNA ORF Clone, Rat, C-HA tag RG81090-CY
    UMP / CMP kinase cDNA ORF Clone, Rat, N-HA tag RG81090-NY
    UMP / CMP kinase cDNA ORF Clone, Rat, C-Myc tag RG81090-CM
    UMP / CMP kinase cDNA ORF Clone, Rat, N-Myc tag RG81090-NM

    UMP / CMP kinase qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    UMP / CMP kinase cDNA ORF Clone in Cloning Vector, Mouse MG5A5239-U
    UMP / CMP kinase cDNA ORF Clone, Mouse, untagged MG5A5239-UT
    UMP / CMP kinase Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG5A5239-ACGLN
    UMP / CMP kinase cDNA ORF Clone, Mouse, C-GFPSpark® tag MG5A5239-ACG
    UMP / CMP kinase cDNA ORF Clone, Mouse, N-GFPSpark® tag MG5A5239-ANG
    UMP / CMP kinase cDNA ORF Clone, Mouse, C-OFPSpark® tag MG5A5239-ACR
    UMP / CMP kinase cDNA ORF Clone, Mouse, N-OFPSpark® tag MG5A5239-ANR
    UMP / CMP kinase cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG5A5239-CF
    UMP / CMP kinase cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG5A5239-NF
    UMP / CMP kinase cDNA ORF Clone, Mouse, C-His tag MG5A5239-CH
    UMP / CMP kinase cDNA ORF Clone, Mouse, N-His tag MG5A5239-NH
    UMP / CMP kinase cDNA ORF Clone, Mouse, C-HA tag MG5A5239-CY
    UMP / CMP kinase cDNA ORF Clone, Mouse, N-HA tag MG5A5239-NY
    UMP / CMP kinase cDNA ORF Clone, Mouse, C-Myc tag MG5A5239-CM
    UMP / CMP kinase cDNA ORF Clone, Mouse, N-Myc tag MG5A5239-NM

    UMP / CMP kinase qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    UMP / CMP kinase cDNA ORF Clone in Cloning Vector, Human HG14258-G
    UMP / CMP kinase cDNA ORF Clone, Human, untagged HG14258-UT
    UMP / CMP kinase cDNA ORF Clone, Human, C-GFPSpark® tag HG14258-ACG
    UMP / CMP kinase Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG14258-ACGLN
    UMP / CMP kinase cDNA ORF Clone, Human, N-GFPSpark® tag HG14258-ANG
    UMP / CMP kinase cDNA ORF Clone, Human, C-OFPSpark® tag HG14258-ACR
    UMP / CMP kinase cDNA ORF Clone, Human, N-OFPSpark® tag HG14258-ANR
    UMP / CMP kinase cDNA ORF Clone, Human, C-DDK (Flag®) tag HG14258-CF
    UMP / CMP kinase cDNA ORF Clone, Human, N-DDK (Flag®) tag HG14258-NF
    UMP / CMP kinase cDNA ORF Clone, Human, C-His tag HG14258-CH
    UMP / CMP kinase cDNA ORF Clone, Human, N-His tag HG14258-NH
    UMP / CMP kinase cDNA ORF Clone, Human, C-HA tag HG14258-CY
    UMP / CMP kinase cDNA ORF Clone, Human, N-HA tag HG14258-NY
    UMP / CMP kinase cDNA ORF Clone, Human, C-Myc tag HG14258-CM
    UMP / CMP kinase cDNA ORF Clone, Human, N-Myc tag HG14258-NM
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