CMBL cDNA ORF Clone, Human, N-His tag

Cat: HG11404-NH
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All CMBL Reagents
CRO Services
Product recommended

All CMBL Reagents
CMBL cDNA ORF Clone, Human, N-His tag General Information
Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
738 bp
Description
Full length Clone DNA of Human carboxymethylenebutenolidase homolog (Pseudomonas) with N terminal His tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-N-His
Tag Sequence
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CMBL cDNA ORF Clone, Human, N-His tag Alternative Names
JS-1 cDNA ORF Clone, Human
CMBL Background Information

Carboxymethylenebutenolidase (CMBL), also known as 4-carboxymethylenebut-2-en-4-olide lactonohydrolase, maleylacetate enol- lactonase, dienelactone hydrolase, and carboxymethylene butenolide hydrolase, is a hydrolase specially belonging to the family of hydrolases. It maily acts on carboxylic ester bonds. CMBL is a human homolog of Pseudomonas dienelactone hydrolase involved in the bacterial halocatechol degradation pathway. The ubiquitous expression of human CMBL gene transcript in various tissues was observed. CMBL was demonstrated to be the primary olmesartan medoxomil (OM) bioactivating enzyme in the liver and intestine. The recombinant human CMBL expressed in mammalian cells was clearly shown to activate OM. The recombinant CMBL also converted other prodrugs having the same ester structure as OM, faropenem medoxomil and lenampicillin, to their active metabolites. CMBL exhibited a unique sensitivity to chemical inhibitors, thus, being distinguishable from other known esterases.  

Full Name
carboxymethylenebutenolidase homolog (Pseudomonas)
References
  • Ishizuka T, et al. (2010) Human Carboxymethylenebutenolidase as a Bioactivating Hydrolase of Olmesartan Medoxomil in Liver and Intestine. The Journal of Biological Chemistry. 285: 11892-902.
  • Schmidt E, et al. (1980) Chemical structure and biodegradability of halogenated aromatic compounds. Conversion of chlorinated muconic acids into maleoylacetic acid. Biochem J. 192 (1): 339-47.
  • CMBL cDNA ORF Clone, Mouse, Related Products

    Product Name Catalog#(PDF)
    CMBL cDNA ORF Clone in Cloning Vector, Mouse MG51405-G
    CMBL cDNA ORF Clone, Mouse, untagged MG51405-UT
    CMBL Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51405-ACGLN
    CMBL cDNA ORF Clone, Mouse, C-GFPSpark® tag MG51405-ACG
    CMBL cDNA ORF Clone, Mouse, N-GFPSpark® tag MG51405-ANG
    CMBL cDNA ORF Clone, Mouse, C-OFPSpark® tag MG51405-ACR
    CMBL cDNA ORF Clone, Mouse, N-OFPSpark® tag MG51405-ANR
    CMBL cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG51405-CF
    CMBL cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG51405-NF
    CMBL cDNA ORF Clone, Mouse, C-His tag MG51405-CH
    CMBL cDNA ORF Clone, Mouse, N-His tag MG51405-NH
    CMBL cDNA ORF Clone, Mouse, C-HA tag MG51405-CY
    CMBL cDNA ORF Clone, Mouse, N-HA tag MG51405-NY
    CMBL cDNA ORF Clone, Mouse, C-Myc tag MG51405-CM
    CMBL cDNA ORF Clone, Mouse, N-Myc tag MG51405-NM
    CMBL qPCR Primer Pairs, Mouse MP201289

    CMBL cDNA ORF Clone, Human, Related Products

    Product Name Catalog#(PDF)
    CMBL cDNA ORF Clone in Cloning Vector, Human HG11404-M
    CMBL cDNA ORF Clone, Human, untagged HG11404-UT
    CMBL cDNA ORF Clone, Human, C-GFPSpark® tag HG11404-ACG
    CMBL Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11404-ACGLN
    CMBL cDNA ORF Clone, Human, N-GFPSpark® tag HG11404-ANG
    CMBL cDNA ORF Clone, Human, C-OFPSpark® tag HG11404-ACR
    CMBL cDNA ORF Clone, Human, N-OFPSpark® tag HG11404-ANR
    CMBL cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11404-CF
    CMBL cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11404-NF
    CMBL cDNA ORF Clone, Human, C-His tag HG11404-CH
    CMBL cDNA ORF Clone, Human, C-HA tag HG11404-CY
    CMBL cDNA ORF Clone, Human, N-HA tag HG11404-NY
    CMBL cDNA ORF Clone, Human, C-Myc tag HG11404-CM
    CMBL cDNA ORF Clone, Human, N-Myc tag HG11404-NM
    CMBL qPCR Primer Pairs, Human HP101282
    info info
    添加购物车成功! 添加购物车失败!