CLPS qPCR Primer Pairs, Human

Cat: HP102315
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CLPS qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
CLPS Background Information

Colipase belongs to the colipase family. Structural studies of the complex and of colipase alone have revealed the functionality of its architecture. It is a small protein with five conserved disulphide bonds. Structural analogies have been recognised between a developmental protein, the pancreatic lipase C-terminal domain, the N-terminal domains of lipoxygenases and the C-terminal domain of alpha-toxin. Colipase can only be detected in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Colipase allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Colipase is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising as active conformation and considerably increasing the overall hydrophobic binding site.

Full Name
colipase, pancreatic
References
  • Davis RC, et al. (1991) Assignment of the human pancreatic colipase gene to chromosome 6p21.1 to pter. Genomics. 10(1):262-5.
  • Lowe ME. (1997) Structure and function of pancreatic lipase and colipase. Annu Rev Nutr. 17: 141-58.
  • Verger R, et al. (1999) Colipase: structure and interaction with pancreatic lipase. Biochim Biophys Acta. 1441(2-3):173-84.
  • CLPS qPCR Primer Pairs, Rhesus, Related Products

    Product Name Catalog#(PDF)
    CLPS cDNA ORF Clone in Cloning Vector, Rhesus CG90523-U
    CLPS cDNA ORF Clone, Rhesus, untagged CG90523-UT
    CLPS cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90523-ACG
    CLPS Lentiviral cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90523-ACGLN
    CLPS cDNA ORF Clone, Rhesus, N-GFPSpark® tag CG90523-ANG
    CLPS cDNA ORF Clone, Rhesus, C-OFPSpark® tag CG90523-ACR
    CLPS cDNA ORF Clone, Rhesus, N-OFPSpark® tag CG90523-ANR
    CLPS cDNA ORF Clone, Rhesus, C-DDK (Flag®) tag CG90523-CF
    CLPS cDNA ORF Clone, Rhesus, N-DDK (Flag®) tag CG90523-NF
    CLPS cDNA ORF Clone, Rhesus, C-His tag CG90523-CH
    CLPS cDNA ORF Clone, Rhesus, N-His tag CG90523-NH
    CLPS cDNA ORF Clone, Rhesus, C-HA tag CG90523-CY
    CLPS cDNA ORF Clone, Rhesus, N-HA tag CG90523-NY
    CLPS cDNA ORF Clone, Rhesus, C-Myc tag CG90523-CM
    CLPS cDNA ORF Clone, Rhesus, N-Myc tag CG90523-NM

    CLPS qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    CLPS cDNA ORF Clone in Cloning Vector, Rat RG80729-U
    CLPS cDNA ORF Clone, Rat, untagged RG80729-UT
    CLPS cDNA ORF Clone, Rat, C-GFPSpark® tag RG80729-ACG
    CLPS Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80729-ACGLN
    CLPS cDNA ORF Clone, Rat, C-OFPSpark® tag RG80729-ACR
    CLPS cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80729-CF
    CLPS cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80729-NF
    CLPS cDNA ORF Clone, Rat, C-His tag RG80729-CH
    CLPS cDNA ORF Clone, Rat, N-His tag RG80729-NH
    CLPS cDNA ORF Clone, Rat, C-HA tag RG80729-CY
    CLPS cDNA ORF Clone, Rat, N-HA tag RG80729-NY
    CLPS cDNA ORF Clone, Rat, C-Myc tag RG80729-CM
    CLPS cDNA ORF Clone, Rat, N-Myc tag RG80729-NM

    CLPS qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    CLPS cDNA ORF Clone in Cloning Vector, Mouse MG53782-U
    CLPS cDNA ORF Clone, Mouse, untagged MG53782-UT
    CLPS Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG53782-ACGLN
    CLPS cDNA ORF Clone, Mouse, C-GFPSpark® tag MG53782-ACG
    CLPS cDNA ORF Clone, Mouse, C-OFPSpark® tag MG53782-ACR
    CLPS cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG53782-CF
    CLPS cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG53782-NF
    CLPS cDNA ORF Clone, Mouse, C-His tag MG53782-CH
    CLPS cDNA ORF Clone, Mouse, N-His tag MG53782-NH
    CLPS cDNA ORF Clone, Mouse, C-HA tag MG53782-CY
    CLPS cDNA ORF Clone, Mouse, N-HA tag MG53782-NY
    CLPS cDNA ORF Clone, Mouse, C-Myc tag MG53782-CM
    CLPS cDNA ORF Clone, Mouse, N-Myc tag MG53782-NM

    CLPS qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    CLPS cDNA ORF Clone in Cloning Vector, Human HG13631-G
    CLPS cDNA ORF Clone, Human, untagged HG13631-UT
    CLPS Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG13631-ACGLN
    CLPS cDNA ORF Clone, Human, C-GFPSpark® tag HG13631-ACG
    CLPS cDNA ORF Clone, Human, C-OFPSpark® tag HG13631-ACR
    CLPS cDNA ORF Clone, Human, C-DDK (Flag®) tag HG13631-CF
    CLPS cDNA ORF Clone, Human, N-DDK (Flag®) tag HG13631-NF
    CLPS cDNA ORF Clone, Human, C-His tag HG13631-CH
    CLPS cDNA ORF Clone, Human, N-His tag HG13631-NH
    CLPS cDNA ORF Clone, Human, C-HA tag HG13631-CY
    CLPS cDNA ORF Clone, Human, N-HA tag HG13631-NY
    CLPS cDNA ORF Clone, Human, C-Myc tag HG13631-CM
    CLPS cDNA ORF Clone, Human, N-Myc tag HG13631-NM
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