|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CLK3 ORF mammalian expression plasmid, C-GFPSpark tag||HG10716-ACG|
|Human CLK3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10716-ACR|
|Human CLK3 ORF mammalian expression plasmid, N-GFPSpark tag||HG10716-ANG|
|Human CLK3 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10716-ANR|
|Human CLK3 ORF mammalian expression plasmid, C-Flag tag||HG10716-CF|
|Human CLK3 ORF mammalian expression plasmid, C-His tag||HG10716-CH|
|Human CLK3 ORF mammalian expression plasmid, C-Myc tag||HG10716-CM|
|Human CLK3 ORF mammalian expression plasmid, C-HA tag||HG10716-CY|
|Human CLK3 ORF mammalian expression plasmid, N-Flag tag||HG10716-NF|
|Human CLK3 ORF mammalian expression plasmid, N-His tag||HG10716-NH|
|Human CLK3 ORF mammalian expression plasmid, N-Myc tag||HG10716-NM|
|Human CLK3 ORF mammalian expression plasmid, N-HA tag||HG10716-NY|
|Human CLK3 Gene cDNA clone plasmid||HG10716-U|
|Human CLK3 natural ORF mammalian expression plasmid||HG10716-UT|
|Learn more about expression Vectors|
Dual specificity protein kinase CLK3, also known as CDC-like kinase 3, and CLK3, is a member of CMGC Ser/Thr protein kinase family and Lammer subfamily. Mammalian CLK is the prototype for a family of dual specificity kinases (termed Lammer kinases) that have been conserved in evolution. CLK family members have shown to interact with, and phosphorylate, serine- and arginine-rich (SR) proteins of the spliceosomal complex, which is a part of the regulatory mechanism that enables the SR proteins to control RNA splicing. The three members of the CLK family of kinases (CLK1, CLK2, and CLK3) have been shown to undergo conserved alternative splicing to generate catalytically active and inactive isoforms. The human CLK2 and CLK3 are found within the nucleus and display dual-specificity kinase activity. The truncated isoforms, hCLK2(T) and hCLK3(T), colocalize with SR proteins in nuclear speckles. CLK3 may play a role in the development and progression of azoospermia.