|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CFH ORF mammalian expression plasmid, C-GFPSpark tag||HG10714-ACG|
|Human CFH ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10714-ACR|
|Human CFH ORF mammalian expression plasmid, C-Flag tag||HG10714-CF|
|Human CFH ORF mammalian expression plasmid, C-His tag||HG10714-CH|
|Human CFH ORF mammalian expression plasmid, C-Myc tag||HG10714-CM|
|Human CFH ORF mammalian expression plasmid, C-HA tag||HG10714-CY|
|Human CFH Gene cDNA clone plasmid||HG10714-G|
|Human CFH ORF mammalian expression plasmid, His tag||HG10714-G-H|
|Human CFH ORF mammalian expression plasmid, N-Flag tag||HG10714-NF|
|Human CFH ORF mammalian expression plasmid, N-His tag||HG10714-NH|
|Human CFH ORF mammalian expression plasmid, N-Myc tag||HG10714-NM|
|Human CFH ORF mammalian expression plasmid, N-HA tag||HG10714-NY|
|Human CFH natural ORF mammalian expression plasmid||HG10714-UT|
|Learn more about expression Vectors|
Complement factor H, also known as H factor 1, and CFH, is a sialic acid containing glycoprotein that plays an integral role in the regulation of the complement-mediated immune system that is involved in microbial defense, immune complex processing, and programmed cell death. Factor H protects host cells from injury resulting from unrestrained complement activation. CFH regulates complement activation on self cells by possessing both cofactor activity for the Factor I mediated C3b cleavage, and decay accelerating activity against the alternative pathway C3 convertase, C3bBb. CFH protects self cells from complement activation but not bacteria/viruses. Due to the central role that CFH plays in the regulation of complement, there are many clinical implications arrising from aberrant CFH activity. Mutations in the Factor H gene are associated with severe and diverse diseases including the rare renal disorders hemolytic uremic syndrome (HUS) and membranoproliferative glomerulonephritis (MPGN) also termed dense deposit disease (DDD), membranoproliferative glomuleronephritis type II or dense deposit disease, as well as the more frequent retinal disease age related macular degeneration (AMD). In addition to its complement regulatory activities, factor H has multiple physiological activities and 1) acts as an extracellular matrix component, 2) binds to cellular receptors of the integrin type, and 3) interacts with a wide selection of ligands, such as the C-reactive protein, thrombospondin, bone sialoprotein, osteopontin, and heparin.