p21/WAF1/CDKN1A cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG11108-CF
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p21/WAF1/CDKN1A cDNA ORF Clone, Human, C-DDK (Flag®) tag General Information
Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
495 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 6 A>T, 93 C>A not causing the amino acid variation.
Description
Full length Clone DNA of Human cyclin-dependent kinase inhibitor 1A (p21, Cip1) with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-FLAG
Restriction Sites
KpnI + XbaI (6kb + 0.53kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
p21/WAF1/CDKN1A cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

p21/WAF1/CDKN1A cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images
Human p21/WAF1/CDKN1A Gene Expression validated Image 1708
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
other Green fluorescent protein / GFP Gene Plasmid Map 5609
p21/WAF1/CDKN1A cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
CAP20 cDNA ORF Clone, Human;CDKN1 cDNA ORF Clone, Human;CIP1 cDNA ORF Clone, Human;MDA-6 cDNA ORF Clone, Human;P21 cDNA ORF Clone, Human;p21CIP1 cDNA ORF Clone, Human;SDI1 cDNA ORF Clone, Human;WAF1 cDNA ORF Clone, Human
p21/WAF1/CDKN1A Background Information
Full Name
cyclin-dependent kinase inhibitor 1A (p21, Cip1)

p21/WAF1/CDKN1A cDNA ORF Clone, Rat, Related Products

Product Name Catalog#(PDF)
p21/WAF1/CDKN1A cDNA ORF Clone in Cloning Vector, Rat RG81956-U
p21/WAF1/CDKN1A Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG81956-ACGLN

p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, Related Products

Product Name Catalog#(PDF)
p21/WAF1/CDKN1A cDNA ORF Clone in Cloning Vector, Mouse MG52793-U
p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, untagged MG52793-UT
p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, C-GFPSpark® tag MG52793-ACG
p21/WAF1/CDKN1A Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG52793-ACGLN
p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, N-GFPSpark® tag MG52793-ANG
p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, C-OFPSpark® tag MG52793-ACR
p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, N-OFPSpark® tag MG52793-ANR
p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG52793-CF
p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG52793-NF
p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, C-His tag MG52793-CH
p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, N-His tag MG52793-NH
p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, C-HA tag MG52793-CY
p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, N-HA tag MG52793-NY
p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, C-Myc tag MG52793-CM
p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, N-Myc tag MG52793-NM

p21/WAF1/CDKN1A cDNA ORF Clone, Human, Related Products

Product Name Catalog#(PDF)
p21/WAF1/CDKN1A cDNA ORF Clone in Cloning Vector, Human HG11108-M
p21/WAF1/CDKN1A cDNA ORF Clone, Human, untagged HG11108-UT
p21/WAF1/CDKN1A cDNA ORF Clone, Human, C-GFPSpark® tag HG11108-ACG
p21/WAF1/CDKN1A Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11108-ACGLN
p21/WAF1/CDKN1A cDNA ORF Clone, Human, N-GFPSpark® tag HG11108-ANG
p21/WAF1/CDKN1A cDNA ORF Clone, Human, C-OFPSpark® tag HG11108-ACR
p21/WAF1/CDKN1A cDNA ORF Clone, Human, N-OFPSpark® tag HG11108-ANR
p21/WAF1/CDKN1A cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11108-NF
p21/WAF1/CDKN1A cDNA ORF Clone, Human, C-His tag HG11108-CH
p21/WAF1/CDKN1A cDNA ORF Clone, Human, N-His tag HG11108-NH
p21/WAF1/CDKN1A cDNA ORF Clone, Human, C-HA tag HG11108-CY
p21/WAF1/CDKN1A cDNA ORF Clone, Human, N-HA tag HG11108-NY
p21/WAF1/CDKN1A cDNA ORF Clone, Human, C-Myc tag HG11108-CM
p21/WAF1/CDKN1A cDNA ORF Clone, Human, N-Myc tag HG11108-NM
p21/WAF1/CDKN1A qPCR Primer Pairs, Human HP101023
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