|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CDK5 ORF mammalian expression plasmid, C-GFPSpark tag||HG10897-ACG|
|Human CDK5 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG10897-ACR|
|Human CDK5 ORF mammalian expression plasmid, N-GFPSpark tag||HG10897-ANG|
|Human CDK5 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||HG10897-ANR|
|Human CDK5 ORF mammalian expression plasmid, C-Flag tag||HG10897-CF|
|Human CDK5 ORF mammalian expression plasmid, C-His tag||HG10897-CH|
|Human CDK5 ORF mammalian expression plasmid, C-Myc tag||HG10897-CM|
|Human CDK5 ORF mammalian expression plasmid, C-HA tag||HG10897-CY|
|Human CDK5 Gene cDNA clone plasmid||HG10897-M|
|Human CDK5 natural ORF mammalian expression plasmid||HG10897-M-N|
|Human CDK5 ORF mammalian expression plasmid, N-Flag tag||HG10897-NF|
|Human CDK5 ORF mammalian expression plasmid, N-His tag||HG10897-NH|
|Human CDK5 ORF mammalian expression plasmid, N-Myc tag||HG10897-NM|
|Human CDK5 ORF mammalian expression plasmid, N-HA tag||HG10897-NY|
|Human CDK5 natural ORF mammalian expression plasmid||HG10897-UT|
|Learn more about expression Vectors|
Cell division protein kinase 5, also known as Cyclin-dependent kinase 5, Serine/threonine-protein kinase PSSALRE, Tau protein kinase II catalytic subunit, TPKII catalytic subunit and CDK5, is a cytoplasm protein which belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2 / CDKX subfamily. Cyclin-dependent kinases (Cdks) are a family of proline-directed Ser/Thr kinases known for their role in the control of cell cycle progression. In 1992, this family was joined by CDK5, which is an atypical member in that it uses its own activators and is multifunctional, playing important regulatory roles in multiple cellular functions. CDK5, unlike other Cdks, is not regulated by cyclins, and its activity is primarily detected in postmitotic neurons in developing and adult nervous systems. CDK5 is activated by association with a neuron-specific activator, p35 or its isoform p39. CDK5 is probably involved in the control of the cell cycle. It interacts with D1 and D3-type G1 cyclins. CDK5 can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. It also interacts with p35 which activates the kinase. CDK5 plays important roles in various neuronal activities, including neuronal migration, synaptic activity, and neuronal cell death.