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Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc tag

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Human CDK1 cDNA Clone Product Information
NCBI RefSeq:NM_001786.4
RefSeq ORF Size:894bp
cDNA Description:Full length Clone DNA of Homo sapiens cell division cycle 2, G1 to S and G2 to M transcript variant 1 with C terminal Myc tag.
Gene Synonym:CDK1, CDC28A, MGC111195, DKFZp686L20222, CDC2
Species:Human
Vector:pCMV3-C-Myc
Plasmid:
Restriction Site:
Tag Sequence:Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Sequence Description:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Myc Tag Info

A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.

The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.

Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc tag on other vectors
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-GFPSpark tagHG10739-ACG$225
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-OFPSpark tagHG10739-ACR$225
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-GFPSpark tagHG10739-ANG$225
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-OFPSpark tagHG10739-ANR$225
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag tagHG10739-CF$195
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-His tagHG10739-CH$195
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc tagHG10739-CM$195
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-HA tagHG10739-CY$195
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone in cloning vectorHG10739-M$75
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag tagHG10739-M-F$195
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmidHG10739-M-N$195
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag tagHG10739-NF$195
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His tagHG10739-NH$195
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Myc tagHG10739-NM$195
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA tagHG10739-NY$195
Human CDC2 Kinase / CDK1 transcript variant 1 Gene ORF cDNA clone expression plasmidHG10739-UT$195
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Background

CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.

References
  • Lee MG, et al. (1987) Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2. Nature. 327(6117):31-5.
  • Enserink JM, et al. (2010) An overview of Cdk1-controlled targets and processes. Cell Division. 5(11): 1-41.
  • Ninomiya-Tsuji J, et al. (1991) Cloning of a human cDNA encoding a CDC2-related kinase by complementation of a budding yeast cdc28 mutation. Proc Natl Acad Sci. 88(20):9006-10.
  • Zhan Q, et al. (1999) Association with Cdc2 and inhibition of Cdc2/Cyclin B1 kinase activity by the p53-regulated protein Gadd45. Oncogene. 18(18):2892-900.
  • Jin S, et al. (2000) The GADD45 inhibition of Cdc2 kinase correlates with GADD45-mediated growth suppression. J Biol Chem. 275(22):16602-8.
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