CD82 qPCR Primer Pairs, Human

Cat: HP101886
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CD82 qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
CD82 qPCR Primer Pairs, Human Alternative Names
4F9 qPCR Primer Pairs, Human;C33 qPCR Primer Pairs, Human;GR15 qPCR Primer Pairs, Human;IA4 qPCR Primer Pairs, Human;KAI-1 qPCR Primer Pairs, Human;KAI1 qPCR Primer Pairs, Human;R2 qPCR Primer Pairs, Human;SAR2 qPCR Primer Pairs, Human;ST6 qPCR Primer Pairs, Human;TSPAN27 qPCR Primer Pairs, Human
CD82 Background Information

CD82, also known as KAI-1, structurally belongs to tetraspanin family while categorised as metastasis suppressor gene on functional grounds. KAI1/CD82 is localized on cell membrane and form interactions with other tetraspanins, integrins and chemokines which are respectively responsible for cell migration, adhesion and signalling. Downregulation of CD82 expression is associated with the advanced stages of many human cancers and correlates with the acquisition of metastatic potential. Recent studies suggest that complex mechanisms underlie CD82 loss of function, including altered transcriptional regulation, splice variant production and post-translational protein modifications, and indicate a central role for CD82 in controlling metastasis as a 'molecular facilitator'. The loss of KAI1/CD82 expression in invasive and metastatic cancers is due to a complex, epigenetic mechanism that probably involves transcription factors such as NFkappaB, p53, and beta-catenin. A loss of KAI1 expression is also associated with the advanced stages of many human malignancies and results in the acquisition of invasive and metastatic capabilities by tumour cells. Thus, KAI1/CD82 is regarded as a wide-spectrum tumor metastasis suppressor.

Full Name
CD82 molecule
References
  • Malik FA, et al. (2009) KAI-1/CD82, the molecule and clinical implication in cancer and cancer metastasis. Histol Histopathol. 24(4): 519-30.
  • Liu WM, et al. (2006) KAI1/CD82, a tumor metastasis suppressor. Cancer Lett. 240(2): 183-94.
  • Tonoli H, et al. (2006) CD82 metastasis suppressor gene: a potential target for new therapeutics? Trends Mol Med. 11(12): 563-70.
  • Jackson P, et al. (2005) KAI1 tetraspanin and metastasis suppressor. Int J Biochem Cell Biol. 37(3): 530-4.
  • CD82 qPCR Primer Pairs, Rhesus, Related Products

    Product Name Catalog#(PDF)
    CD82 cDNA ORF Clone in Cloning Vector, Rhesus CG90202-G
    CD82 cDNA ORF Clone, Rhesus, untagged CG90202-UT
    CD82 Lentiviral cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90202-ACGLN
    CD82 cDNA ORF Clone, Rhesus, C-GFPSpark® tag CG90202-ACG
    CD82 cDNA ORF Clone, Rhesus, C-OFPSpark® tag CG90202-ACR
    CD82 cDNA ORF Clone, Rhesus, C-DDK (Flag®) tag CG90202-CF
    CD82 cDNA ORF Clone, Rhesus, N-DDK (Flag®) tag CG90202-NF
    CD82 cDNA ORF Clone, Rhesus, C-His tag CG90202-CH
    CD82 cDNA ORF Clone, Rhesus, N-His tag CG90202-NH
    CD82 cDNA ORF Clone, Rhesus, C-HA tag CG90202-CY
    CD82 cDNA ORF Clone, Rhesus, N-HA tag CG90202-NY
    CD82 cDNA ORF Clone, Rhesus, C-Myc tag CG90202-CM
    CD82 cDNA ORF Clone, Rhesus, N-Myc tag CG90202-NM

    CD82 qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    CD82 cDNA ORF Clone in Cloning Vector, Rat RG80332-G
    CD82 cDNA ORF Clone, Rat, untagged RG80332-UT
    CD82 Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80332-ACGLN
    CD82 cDNA ORF Clone, Rat, C-GFPSpark® tag RG80332-ACG
    CD82 cDNA ORF Clone, Rat, C-OFPSpark® tag RG80332-ACR
    CD82 cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80332-CF
    CD82 cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80332-NF
    CD82 cDNA ORF Clone, Rat, C-His tag RG80332-CH
    CD82 cDNA ORF Clone, Rat, N-His tag RG80332-NH
    CD82 cDNA ORF Clone, Rat, C-HA tag RG80332-CY
    CD82 cDNA ORF Clone, Rat, N-HA tag RG80332-NY
    CD82 cDNA ORF Clone, Rat, C-Myc tag RG80332-CM
    CD82 cDNA ORF Clone, Rat, N-Myc tag RG80332-NM

    CD82 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    CD82 cDNA ORF Clone in Cloning Vector, Mouse MG50744-G
    CD82 cDNA ORF Clone, Mouse, untagged MG50744-UT
    CD82 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50744-ACGLN
    CD82 cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50744-ACG
    CD82 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50744-ACR
    CD82 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50744-CF
    CD82 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50744-NF
    CD82 cDNA ORF Clone, Mouse, C-His tag MG50744-CH
    CD82 cDNA ORF Clone, Mouse, N-His tag MG50744-NH
    CD82 cDNA ORF Clone, Mouse, C-HA tag MG50744-CY
    CD82 cDNA ORF Clone, Mouse, N-HA tag MG50744-NY
    CD82 cDNA ORF Clone, Mouse, C-Myc tag MG50744-CM
    CD82 cDNA ORF Clone, Mouse, N-Myc tag MG50744-NM

    CD82 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    CD82 cDNA ORF Clone in Cloning Vector, Human HG12275-G
    CD82 cDNA ORF Clone, Human, untagged HG12275-UT
    CD82 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG12275-ACGLN
    CD82 cDNA ORF Clone, Human, C-GFPSpark® tag HG12275-ACG
    CD82 cDNA ORF Clone, Human, C-OFPSpark® tag HG12275-ACR
    CD82 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG12275-CF
    CD82 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG12275-NF
    CD82 cDNA ORF Clone, Human, C-His tag HG12275-CH
    CD82 cDNA ORF Clone, Human, N-His tag HG12275-NH
    CD82 cDNA ORF Clone, Human, C-HA tag HG12275-CY
    CD82 cDNA ORF Clone, Human, N-HA tag HG12275-NY
    CD82 cDNA ORF Clone, Human, C-Myc tag HG12275-CM
    CD82 cDNA ORF Clone, Human, N-Myc tag HG12275-NM
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