|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by Western blotting.
The peptide sequence of the myc-tag is: N-EQKLISEEDL-C (1202 Da). It can be fused to the C-terminus and the N-terminus of a protein. It is advisable not to fuse the tag directly behind the signal peptide of a secretory protein, since it can interfere with translocation into the secretory pathway.
|Human CD81 ORF mammalian expression plasmid, C-GFPSpark tag||HG14244-ACG|
|Human CD81 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||HG14244-ACR|
|Human CD81 ORF mammalian expression plasmid, C-Flag tag||HG14244-CF|
|Human CD81 ORF mammalian expression plasmid, C-His tag||HG14244-CH|
|Human CD81 ORF mammalian expression plasmid, C-Myc tag||HG14244-CM|
|Human CD81 ORF mammalian expression plasmid, C-HA tag||HG14244-CY|
|Human CD81 Gene cDNA clone plasmid||HG14244-G|
|Human CD81 ORF mammalian expression plasmid, N-Flag tag||HG14244-NF|
|Human CD81 ORF mammalian expression plasmid, N-His tag||HG14244-NH|
|Human CD81 ORF mammalian expression plasmid, N-Myc tag||HG14244-NM|
|Human CD81 ORF mammalian expression plasmid, N-HA tag||HG14244-NY|
|Human CD81 natural ORF mammalian expression plasmid||HG14244-UT|
|Learn more about expression Vectors|
CD81, also known as TAPA-1, belongs to the transmembrane 4 superfamily, also known as the tetraspanin family. Members of this family mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.CD81 is a widely expressed cell-surface protein involved in an astonishing variety of biologic responses. It is related to adhesion, morphology, activation, proliferation, and differentiation of B, T, and other cells. On B cells CD81 is part of a complex with CD21, CD19, and Leu13. This complex reduces the threshold for B cell activation via the B cell receptor by bridging Ag specific recognition and CD21-mediated complement recognition.