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Human CD3 (humanized OKT3) Antibody

  • Human CD3 (humanized OKT3) Antibody, Agonist Activity
  • Human CD3 (humanized OKT3) Antibody, Agonist Activity
  • Human CD3 (humanized OKT3) Antibody
  • Human CD3 (humanized OKT3) Antibody
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Human CD3E Antibody Product Information
Immunogen:
Clone ID:H001
Ig Type:Human IgG4
Concentration:
Endotoxin:< 0.5 EU/mg
Formulation:0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
Preparation:Human CD3 (humanized OKT3) Antibody is a humanized antibody of Orthoclone OKT3. Orthoclone OKT3 is an immunosuppressant drug given to reduce acute rejection in patients with organ transplants. It is a monoclonal antibody targeted at the CD3 receptor, a membrane protein on the surface of T cells. It was the first monoclonal antibody to be approved for clinical use in humans.
Human CD3E Antibody FC Application Image
Human CD3 (humanized OKT3) Antibody
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Caption:
The staining effect of Human CD3 (humanized OKT3) antibody to CD3 of Human Peripheral Blood Lymphocytes by Flow cytometric analysis.
A: Lymphocytes were stained with PE-conjugated human CD3 (humanized OKT3) antibody.
B: The binding effect of PE-conjugated humanized OKT3 antibody(Red histograms) in the lymphocytes can be blocked by pre-incubation of the CD3 antibody (Miltenyi Biotec, 130-093-387) for 20min (blue histograms).

Human CD3 (humanized OKT3) Antibody
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Caption:
CD3+ T cell staining of lymphocytes using Human CD3 (humanized OKT3) antibody by Flow cytometric analysis.
A: Lymphocytes were stained with both PE-conjugated CD3(humanized OKT3) antibody and OKT3-FITC-eBioscience (eBioscience, 11-0037-41).
B: Lymphocytes were stained with PE-conjugated humanized OKT3 antibody and CD19-APC (BD, 555415).

Human CD3E Antibody Neutralization Application Image
Human CD3 (humanized OKT3) Antibody, Agonist Activity
[Click to enlarge image]
Caption:
Effect of Human CD3 (humanized OKT3) antibody on the proliferation and IFN-gamma secretion of Peripheral blood mononuclear cells (PBMC). The isolated PBMC (1e5/well) were cultured for 4 days in the 96-well cell plates coated with serial dilutions of human CD3 (humanized OKT3) antibody.
A. The cell proliferation level was detected using MTT detection;
A. The cell proliferation level was detected using MTT detection;
B. IFN-gamma secretion in the medium was measured by IFN-gamma Quantitative ELISA Kit.

Human CD3 (humanized OKT3) Antibody, Agonist Activity
[Click to enlarge image]
Caption:
Effect of Human CD3 (humanized OKT3) antibody on the proliferation and IFN-gamma secretion of CD4+ T cell.
The isolated CD4+ T cells(1e5/well) were cultured for 4 days in the 96-well cell plates coated with serial dilutions of CD3 (humanized OKT3) antibody.
A. The cell proliferation level was detected using MTT detection;
B. IFN-gamma secretion in the medium was measured by IFN-gamma Quantitative ELISA Kit.

Other CD3E Antibody Products
Immunochemical staining of human CD40 in human tonsil with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
CD3e/CD3 epsilon Background

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. In the CD3 epsilon mutant mice, all stages of T cell development and activation that are TCR-dependent were impaired, but not eliminated, including activation of mature naïve T cells with the MHCII presented superantigen, staphylococcal enterotoxin B, or with a strong TCR cross-linking antibody specific for either TCR-Cbeta or CD3 epsilon. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.

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Human CD3e/CD3 epsilon References
  • Fischer A, et al. (2005) CD3 deficiencies. Curr Opin Allergy Clin Immunol. 5(6): 491-5.
  • Wang Y, et al. (2009) A conserved CXXC motif in CD3epsilon is critical for T cell development and TCR signaling. PLoS Biol. 7(12): e1000253.
  • Martnez-Martn N, et al. (2009) Cooperativity between T cell receptor complexes revealed by conformational mutants of CD3epsilon. Sci Signal. 2(83): ra43.
  • Deford-Watts LM, et al. (2009) The cytoplasmic tail of the T cell receptor CD3 epsilon subunit contains a phospholipid-binding motif that regulates T cell functions. J Immunol. 183(2): 1055-64.
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"