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Human CD28 Antibody Product Information
Immunogen:
Clone ID:H001
Ig Type:Human IgG2
Concentration:
Endotoxin:< 0.5 EU/mg
Formulation:0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
Preparation:
Human CD28 Antibody FC Application Image
Human CD28 Antibody
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Caption:
The staining effect of Human CD28 antibody to CD28 of Human Peripheral Blood Lymphocytes by Flow cytometric analysis.
A: Lymphocytes were stained with PE-conjugated humanized CD28 antibody.
B: The binding effect of PE-conjugated human CD28 antibody(Red histograms) in the lymphocytes can be blocked by pre-incubation of the CD28 antibody (BD, 555725) for 20min (blue histograms).

Human CD28 Antibody
[Click to enlarge image]
Caption:
CD28+ T cell staining of lymphocytes using human CD28 antibody by Flow cytometric analysis.
Lymphocytes were stained with PE-conjugated humanized CD28 antibody and CD19-APC (BD, 555415).

Human CD28 Antibody Neutralization Application Image
Human CD28 Antibody, Agonist Activity
[Click to enlarge image]
Caption:
Effect of human CD28 antibody on stimulating IL-2 secretion of CD4 T cells co-activated with CD3 antibody. The isolated CD4+ T cells (1e5/well) were cultured in the 96-well cell plates pre-coated with 2 μg/mL human CD3 (humanized OKT3) antibody (10977-H001) in the culture medium containing serial dilutions of CD28 antibody. 48 hours later, IL-2 secretion in the medium was measured by IL-2 Quantitative ELISA Kit.
Other CD28 Antibody Products
Immunochemical staining of human CD40 in human tonsil with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
CD28/TP44 Background

CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IHC Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Targets   Co-stimulatory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Human CD28/TP44 References
  • Keir ME, et al. (2005) The B7/CD28 costimulatory family in autoimmunity. Immunol Rev. 204: 128-43.
  • Sansom DM, et al. (2006) The role of CD28 and cytotoxic T-lymphocyte antigen-4 (CTLA-4) in regulatory T-cell biology. Immunol Rev. 212: 131-48.
  • Bjrgo E, et al. (2010) Novel mechanism of signaling by CD28. Immunol Lett. 129(1): 1-6.
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    Catalog: 11524-H001-100
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"