RP105 qPCR Primer Pairs, Human

Cat: HP101252
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RP105 qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
RP105 qPCR Primer Pairs, Human Alternative Names
LY64 qPCR Primer Pairs, Human;Ly78 qPCR Primer Pairs, Human;RP105 qPCR Primer Pairs, Human
RP105 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD180, also known as RP105, is a B-cell surface molecule belonging to the family of pathogen receptors, Toll-like receptors (TLR). CD180 has an extracellular leucine-rich repeats and a short cytoplasmic tail. CD180 / RP105 interact with an extracellular molecule named MD1 and then together form the cell surface receptor complex RP105 / MD1 which induces B-cell activation in humans and mice, leading to proliferation and up-regulation of a costimulatory molecule, B7.2 / CD86. CD180 / RP105 also has a role in LPS response because B cells lacking RP105 show hyporesponsiveness to LPS.

Full Name
CD180 molecule
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Nagai Y, et al. (2002) Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide. Journal of the American society of hematology. 99(5): 1699-705.
  • RP105 qPCR Primer Pairs, Rhesus, Related Products

    Product Name Catalog#(PDF)
    RP105 cDNA ORF Clone in Cloning Vector, Rhesus CG90222-G
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    RP105 cDNA ORF Clone, Rhesus, N-His tag CG90222-NH
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    RP105 qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    RP105 cDNA ORF Clone in Cloning Vector, Mouse MG50491-M
    RP105 cDNA ORF Clone, Mouse, untagged MG50491-UT
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    RP105 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50491-ACGLN
    RP105 cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50491-ACR
    RP105 cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50491-CF
    RP105 cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50491-NF
    RP105 cDNA ORF Clone, Mouse, C-His tag MG50491-CH
    RP105 cDNA ORF Clone, Mouse, N-His tag MG50491-NH
    RP105 cDNA ORF Clone, Mouse, C-HA tag MG50491-CY
    RP105 cDNA ORF Clone, Mouse, N-HA tag MG50491-NY
    RP105 cDNA ORF Clone, Mouse, C-Myc tag MG50491-CM
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    RP105 qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    RP105 cDNA ORF Clone in Cloning Vector, Human HG11370-M
    RP105 cDNA ORF Clone, Human, untagged HG11370-UT
    RP105 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11370-ACGLN
    RP105 cDNA ORF Clone, Human, C-GFPSpark® tag HG11370-ACG
    RP105 cDNA ORF Clone, Human, C-OFPSpark® tag HG11370-ACR
    RP105 cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11370-CF
    RP105 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11370-NF
    RP105 cDNA ORF Clone, Human, C-His tag HG11370-CH
    RP105 cDNA ORF Clone, Human, N-His tag HG11370-NH
    RP105 cDNA ORF Clone, Human, C-HA tag HG11370-CY
    RP105 cDNA ORF Clone, Human, N-HA tag HG11370-NY
    RP105 cDNA ORF Clone, Human, C-Myc tag HG11370-CM
    RP105 cDNA ORF Clone, Human, N-Myc tag HG11370-NM
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