CD177 cDNA ORF Clone, Human, C-DDK (Flag®) tag

Cat: HG14501-CF
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CD177 cDNA ORF Clone, Human, C-DDK (Flag®) tag General Information
Gene
Species
Human
NCBI Ref Seq
RefSeq ORF Size
1314 bp
Sequence Description
Identical with the Gene Bank Ref. ID sequence.
Description
Full length Clone DNA of Human CD177 molecule with C terminal Flag tag.
Plasmid
Promoter
Enhanced CMV promoter
Vector
pCMV3-C-FLAG
Restriction Sites
KpnI + XbaI (6kb + 1.35kb)
Tag Sequence
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequencing Primers
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
Quality Control
The plasmid is confirmed by full-length sequencing.
Screening
Antibiotic in E.coli
Kanamycin
Antibiotic in Mammalian cell
Hygromycin
Application
Stable or Transient mammalian expression
Storage & Shipping
Shipping
Each tube contains lyophilized plasmid.
Storage
The lyophilized plasmid can be stored at ambient temperature for three months.
CD177 cDNA ORF Neucleotide Sequence and Amino Acid Sequence Information

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CD177 cDNA ORF Clone, Human, C-DDK (Flag®) tag Validated Images
Human CD177 / PRV-1 / PRV1 Gene Expression validated Image 1984
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
other Green fluorescent protein / GFP Gene Plasmid Map 5609
CD177 cDNA ORF Clone, Human, C-DDK (Flag®) tag Alternative Names
HNA-2a cDNA ORF Clone, Human;HNA2A cDNA ORF Clone, Human;NB1 cDNA ORF Clone, Human;NB1GP cDNA ORF Clone, Human;PRV-1 cDNA ORF Clone, Human;PRV1 cDNA ORF Clone, Human
CD177 Background Information
Full Name
CD177 molecule

CD177 cDNA ORF Clone, Ferret, Related Products

Product Name Catalog#(PDF)
CD177 cDNA ORF Clone in Cloning Vector, Ferret FG60079-G
CD177 cDNA ORF Clone, Ferret, untagged FG60079-UT
CD177 cDNA ORF Clone, Ferret, C-GFPSpark® tag FG60079-ACG
CD177 cDNA ORF Clone, Ferret, C-OFPSpark® tag FG60079-ACR
CD177 cDNA ORF Clone, Ferret, C-DDK (Flag®) tag FG60079-CF
CD177 cDNA ORF Clone, Ferret, N-DDK (Flag®) tag FG60079-NF
CD177 cDNA ORF Clone, Ferret, C-His tag FG60079-CH
CD177 cDNA ORF Clone, Ferret, N-His tag FG60079-NH
CD177 cDNA ORF Clone, Ferret, C-HA tag FG60079-CY
CD177 cDNA ORF Clone, Ferret, N-HA tag FG60079-NY
CD177 cDNA ORF Clone, Ferret, C-Myc tag FG60079-CM
CD177 cDNA ORF Clone, Ferret, N-Myc tag FG60079-NM

CD177 cDNA ORF Clone, Mouse, Related Products

Product Name Catalog#(PDF)
CD177 cDNA ORF Clone in Cloning Vector, Mouse MG54630-U
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CD177 cDNA ORF Clone, Human, Related Products

Product Name Catalog#(PDF)
CD177 cDNA ORF Clone in Cloning Vector, Human HG14501-G
CD177 cDNA ORF Clone, Human, untagged HG14501-UT
CD177 Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG14501-ACGLN
CD177 cDNA ORF Clone, Human, C-GFPSpark® tag HG14501-ACG
CD177 cDNA ORF Clone, Human, C-OFPSpark® tag HG14501-ACR
CD177 cDNA ORF Clone, Human, N-DDK (Flag®) tag HG14501-NF
CD177 cDNA ORF Clone, Human, C-His tag HG14501-CH
CD177 cDNA ORF Clone, Human, N-His tag HG14501-NH
CD177 cDNA ORF Clone, Human, C-HA tag HG14501-CY
CD177 cDNA ORF Clone, Human, N-HA tag HG14501-NY
CD177 cDNA ORF Clone, Human, C-Myc tag HG14501-CM
CD177 cDNA ORF Clone, Human, N-Myc tag HG14501-NM
CD177 qPCR Primer Pairs, Human HP103148
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