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Human C5a anaphylatoxin receptor 1 Gene ORF cDNA clone expression plasmid, C-His tag

  • other Green fluorescent protein / GFP Gene Plasmid Map 5610
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Human C5AR1 cDNA Clone Product Information
NCBI RefSeq:BC008982
RefSeq ORF Size:1098 bp
cDNA Description:Full length Clone DNA of Homo sapiens complement component 5a receptor 1 with C terminal His tag.
Gene Synonym:C5A, C5AR, C5R1, CD88, C5AR1
Species:Human
Vector:pCMV3-C-His
Plasmid:pCMV3-C5AR1-His
Restriction Site:KpnI + XbaI(6kb+1.1kb)
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
( We provide with C5AR1 qPCR primers for gene expression analysis, HP101809 )
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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Catalog: HG12154-CH
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