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Human BUB3 Gene ORF cDNA clone expression plasmid, C-His tag

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Human BUB3 cDNA Clone Product Information
NCBI RefSeq:NM_004725.3
RefSeq ORF Size:987bp
cDNA Description:Full length Clone DNA of Homo sapiens POT1 protection of telomeres 1 homolog with C terminal His tag.
Gene Synonym:hPot1, DKFZp586D211, POT1
Species:Human
Vector:pCMV3-C-His
Plasmid:pCMV3-BUB3-His
Restriction Site:HindIII + XbaI (6kb + 1.03kb)
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Human BUB3 Gene Plasmid Map
Human BUB3 natural ORF mammalian expression plasmid, C-His tag
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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