BLMH qPCR Primer Pairs, Human

Cat: HP101218
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BLMH qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
BLMH qPCR Primer Pairs, Human Alternative Names
BH qPCR Primer Pairs, Human;BMH qPCR Primer Pairs, Human
BLMH Background Information

The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different types of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.

Full Name
bleomycin hydrolase
Research Areas
References
  • Takeda A, et al. (1996) Cloning and Analysis of cDNA Encoding Rat Bleomycin Hydrolase, a DNA-Binding Cysteine Protease. J Biochem. 120 (2): 353-9.
  • Lefterov IM, et al. (2000) Human bleomycin hydrolase regulates the secretion of amyloid precursor protein. The FASEB Journal. 14(12): 1837-47.
  • Brmme D, et al. (1996) Human Bleomycin Hydrolase: Molecular Cloning, Sequencing, Functional Expression, and Enzymatic Characterization. Biochemistry. 35 (21): 6706-14.
  • BLMH qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    BLMH cDNA ORF Clone in Cloning Vector, Rat RG80859-U
    BLMH cDNA ORF Clone, Rat, untagged RG80859-UT
    BLMH Lentiviral cDNA ORF Clone, Rat, C-GFPSpark® tag RG80859-ACGLN
    BLMH cDNA ORF Clone, Rat, C-GFPSpark® tag RG80859-ACG
    BLMH cDNA ORF Clone, Rat, N-GFPSpark® tag RG80859-ANG
    BLMH cDNA ORF Clone, Rat, C-OFPSpark® tag RG80859-ACR
    BLMH cDNA ORF Clone, Rat, N-OFPSpark® tag RG80859-ANR
    BLMH cDNA ORF Clone, Rat, C-DDK (Flag®) tag RG80859-CF
    BLMH cDNA ORF Clone, Rat, N-DDK (Flag®) tag RG80859-NF
    BLMH cDNA ORF Clone, Rat, C-His tag RG80859-CH
    BLMH cDNA ORF Clone, Rat, N-His tag RG80859-NH
    BLMH cDNA ORF Clone, Rat, C-HA tag RG80859-CY
    BLMH cDNA ORF Clone, Rat, N-HA tag RG80859-NY
    BLMH cDNA ORF Clone, Rat, C-Myc tag RG80859-CM
    BLMH cDNA ORF Clone, Rat, N-Myc tag RG80859-NM

    BLMH qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    BLMH cDNA ORF Clone in Cloning Vector, Mouse MG50668-M
    BLMH cDNA ORF Clone, Mouse, untagged MG50668-UT
    BLMH cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50668-ACG
    BLMH Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50668-ACGLN
    BLMH cDNA ORF Clone, Mouse, N-GFPSpark® tag MG50668-ANG
    BLMH cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50668-ACR
    BLMH cDNA ORF Clone, Mouse, N-OFPSpark® tag MG50668-ANR
    BLMH cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50668-CF
    BLMH cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50668-NF
    BLMH cDNA ORF Clone, Mouse, C-His tag MG50668-CH
    BLMH cDNA ORF Clone, Mouse, N-His tag MG50668-NH
    BLMH cDNA ORF Clone, Mouse, C-HA tag MG50668-CY
    BLMH cDNA ORF Clone, Mouse, N-HA tag MG50668-NY
    BLMH cDNA ORF Clone, Mouse, C-Myc tag MG50668-CM
    BLMH cDNA ORF Clone, Mouse, N-Myc tag MG50668-NM

    BLMH qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    BLMH cDNA ORF Clone in Cloning Vector, Human HG11333-M
    BLMH cDNA ORF Clone, Human, untagged HG11333-UT
    BLMH Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11333-ACGLN
    BLMH cDNA ORF Clone, Human, C-GFPSpark® tag HG11333-ACG
    BLMH cDNA ORF Clone, Human, N-GFPSpark® tag HG11333-ANG
    BLMH cDNA ORF Clone, Human, C-OFPSpark® tag HG11333-ACR
    BLMH cDNA ORF Clone, Human, N-OFPSpark® tag HG11333-ANR
    BLMH cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11333-CF
    BLMH cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11333-NF
    BLMH cDNA ORF Clone, Human, C-His tag HG11333-CH
    BLMH cDNA ORF Clone, Human, N-His tag HG11333-NH
    BLMH cDNA ORF Clone, Human, C-HA tag HG11333-CY
    BLMH cDNA ORF Clone, Human, N-HA tag HG11333-NY
    BLMH cDNA ORF Clone, Human, C-Myc tag HG11333-CM
    BLMH cDNA ORF Clone, Human, N-Myc tag HG11333-NM
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