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Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag tag

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Human BIRC5 cDNA Clone Product Information
NCBI RefSeq:NM_001168.2
RefSeq ORF Size:429bp
cDNA Description:Full length Clone DNA of Homo sapiens baculoviral IAP repeat-containing 5, transcript variant 1 with C terminal Flag tag.
Gene Synonym:API4, EPR-1
Species:Human
Vector:pCMV3-C-FLAG
Plasmid:pCMV3-BIRC5-Flag
Restriction Site:KpnI + XbaI (6kb + 0.47kb)
Tag Sequence:FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
Human BIRC5 Gene Plasmid Map
Human BIRC5 transcript variant 1 natural ORF mammalian expression plasmid, C-Flag tag
FLAG Tag Info

FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.

A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.

The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or Myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.

Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag tag on other vectors
Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-GFPSpark tagHG10356-ACG$225
Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-OFPSpark tagHG10356-ACR$225
Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-GFPSpark tagHG10356-ANG$225
Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-OFPSpark tagHG10356-ANR$225
Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Flag tagHG10356-CF$195
Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-His tagHG10356-CH$195
Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-Myc tagHG10356-CM$195
Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmid, C-HA tagHG10356-CY$195
Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone in cloning vectorHG10356-M$75
Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmidHG10356-M-N$195
Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Flag tagHG10356-NF$195
Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-His tagHG10356-NH$195
Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-Myc tagHG10356-NM$195
Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmid, N-HA tagHG10356-NY$195
Human Survivin/BIRC5/API4 transcript variant 1 Gene ORF cDNA clone expression plasmidHG10356-UT$195
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Background

BIRC5, also known as Survivin and EPR-1, is a member of the IAP family. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but BIRC5 has only a single BIR domain. It is expressed cell cycle-dependently and highly expressed at mitosis. As a multitasking protein, BIRC5 has dual roles in promoting cell proliferation and preventing apoptosis. Survivin is a component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis. Survivin acts as an important regulator of the localization of this complex. It may counteract a default induction of apoptosis in G2/M phase.

References
  • Altieri DC. 1994, J Biol Chem. 269 (5): 3139-42.
  • Bouchard BA. et al., 2002, Thromb Haemost. 86 (4): 1133-5.
  • Yao XQ. et al., 2004, World J Gastroenterol. 10 (9): 1262-7.
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