ARSA qPCR Primer Pairs, Human

Cat: HP100478
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ARSA qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
PCR Size:
164bp
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
ARSA qPCR Primer Pairs, Human Alternative Names
MLD qPCR Primer Pairs, Human
ARSA Background Information

Arylsulfatase A (ARSA) is synthesized as a 52KDa lysosomal enzyme. It is a member of the sulfatase family that is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet. Arylsulfatase A is activated by a required co- or posttranslational modification with the oxidation of cysteine to formylglycine. Metachromatic leukodystrophy (MLD) is a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms caused by the deficiency of Arylsulfatase A. Deficiency of this enzyme is also found in apparently healthy individuals, a condition for which the term pseudodeficiency is introduced. ARSA forms dimers after receiving three N-linked oligosaccharides in the endoplasmic reticulum, and then the dimers are transported to the Golgi where they receive mannose 6-phosphate recognition markers. And thus, ARSA is transported and delivered to dense lysosomes in a mannose 6-phosphate receptor-dependent manner. It has been shown that within the lysosomes, the ARSA dimers can oligomerize to an octamer in a pH-dependent manner. The ARSA deficiency leads to metachromatic leucodystrophy (MLD), a lysosomal storage disorder associated with severe and progressive demyelination in he central and peripheral nervous system. Additionally, the serum level of arylsulfatase A might be helpful in diagnosis of lung and central nervous system cancer.

Full Name
arylsulfatase A
References
  • Laidler PM. (1991) Arylsulfatase A--physico-chemical properties and the use of enzyme radioimmunoassay in medical diagnosis Folia Med Cracov. 32(3-4): 149-68.
  • Jean S, et al. (2006) Ethanol decreases rat hepatic arylsulfatase A activity levels. Alcohol Clin Exp Res. 30(11): 1950-5.
  • ARSA qPCR Primer Pairs, Mouse, Related Products

    Product Name Catalog#(PDF)
    ARSA cDNA ORF Clone in Cloning Vector, Mouse MG50018-M
    ARSA cDNA ORF Clone, Mouse, untagged MG50018-UT
    ARSA Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50018-ACGLN
    ARSA cDNA ORF Clone, Mouse, C-GFPSpark® tag MG50018-ACG
    ARSA cDNA ORF Clone, Mouse, C-OFPSpark® tag MG50018-ACR
    ARSA cDNA ORF Clone, Mouse, C-DDK (Flag®) tag MG50018-CF
    ARSA cDNA ORF Clone, Mouse, N-DDK (Flag®) tag MG50018-NF
    ARSA cDNA ORF Clone, Mouse, C-His tag MG50018-CH
    ARSA cDNA ORF Clone, Mouse, N-His tag MG50018-NH
    ARSA cDNA ORF Clone, Mouse, C-HA tag MG50018-CY
    ARSA cDNA ORF Clone, Mouse, N-HA tag MG50018-NY
    ARSA cDNA ORF Clone, Mouse, C-Myc tag MG50018-CM
    ARSA cDNA ORF Clone, Mouse, N-Myc tag MG50018-NM

    ARSA qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    ARSA cDNA ORF Clone in Cloning Vector, Human HG10449-M
    ARSA cDNA ORF Clone, Human, untagged HG10449-UT
    ARSA cDNA ORF Clone, Human, C-GFPSpark® tag HG10449-ACG
    ARSA Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG10449-ACGLN
    ARSA cDNA ORF Clone, Human, C-OFPSpark® tag HG10449-ACR
    ARSA cDNA ORF Clone, Human, C-DDK (Flag®) tag HG10449-CF
    ARSA cDNA ORF Clone, Human, N-DDK (Flag®) tag HG10449-NF
    ARSA cDNA ORF Clone, Human, C-His tag HG10449-CH
    ARSA cDNA ORF Clone, Human, N-His tag HG10449-NH
    ARSA cDNA ORF Clone, Human, C-HA tag HG10449-CY
    ARSA cDNA ORF Clone, Human, N-HA tag HG10449-NY
    ARSA cDNA ORF Clone, Human, C-Myc tag HG10449-CM
    ARSA cDNA ORF Clone, Human, N-Myc tag HG10449-NM
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