Adenylosuccinate Lyase qPCR Primer Pairs, Human

Cat: HP101165
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Adenylosuccinate Lyase qPCR Primer Pairs, Human General Information
Target Details
Species:
Human
Product Details
Oligo-Type:
qPCR Primers
Component:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCR Primer Description:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
Application & Quality
Application:
SYBR® Green-based quantitative real-time PCR (qPCR).
Quality Control:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
Storage & Shipping
Shipping:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
Storage:
The lyophilized product is stable for one year from date of receipt when stored at -20℃. The suspended product is stable for six months from date of receipt when stored at -20℃.

***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***

Features and Advantages
Unique Primer Design
To avoid genomic DNA amplification, at least one primer is designed crosses the junction of exons according to the conserved region of a specific gene with all variants.
Strict Validation Process
Confirmed in positive organizations; screened the primer with high specificity and high sensitivity.
Uniform PCR conditions, Saving time and cost
~100% amplification curve, ensuring the accuracy of the RNA quantitative
Adenylosuccinate Lyase qPCR Primer Pairs, Human Alternative Names
AMPS qPCR Primer Pairs, Human;ASASE qPCR Primer Pairs, Human;ASL qPCR Primer Pairs, Human
Adenylosuccinate Lyase Background Information

Adenylosuccinate lyase, also known as adenylosuccinase, ADSL or ASL, is an enzyme implicated in the reaction of adenylosuccinat converting to AMP and fumarate as part of the purine nucleotide cycle. The two substates of adenylosuccinate lyase (ADSL) are dephosphorylated derivatives of SAICA ribotide (SAICAR) and adenylosuccinate (S-AMP), which catalyzes an important reaction in the de novo pathway of purine biosynthesis. ADSL catalyzes two distinct reactions in the synthesis of purine nucleotides, both of which involve the _-elimination of fumarate to produce either aminoimidazole carboxamide ribotide from SAICAR or AMP from S-AMP. The Adenylosuccinate lyase deficiency is a rare autosomal recessive metabolic disorder characterized by the present of SAICA riboside and succinyladenosine (S-Ado). ADSL defect in different patients is often caused by different mutations to the enzyme.

Full Name
adenylosuccinate lyase
References
  • Nassogne M, et al. (2000) Adenylosuccinase deficiency: an unusual cause of early-onset epilepsy associated with acquired microcephaly. Brain and development. 22 (6): 383-6.
  • Sivendran S, et al. (2004) Two novel mutant human adenylosuccinate lyases (ASLs) associated with autism and characterization of the equivalent mutant Bacillus subtilis ASL. J Biol Chem. 279 (51): 53789-97.
  • Lee TT, et al. (1999) His68 and His141 are critical contributors to the intersubunit catalytic site of adenylosuccinate lyase of Bacillus subtilis. Biochemistry. 38 (1): 22-32.
  • Adenylosuccinate Lyase qPCR Primer Pairs, Rat, Related Products

    Product Name Catalog#(PDF)
    Adenylosuccinate Lyase cDNA ORF Clone in Cloning Vector, Rat RG81324-G
    Adenylosuccinate Lyase cDNA ORF Clone, Rat, untagged RG81324-UT
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    Adenylosuccinate Lyase cDNA ORF Clone, Rat, C-His tag RG81324-CH
    Adenylosuccinate Lyase cDNA ORF Clone, Rat, N-His tag RG81324-NH
    Adenylosuccinate Lyase cDNA ORF Clone, Rat, C-HA tag RG81324-CY
    Adenylosuccinate Lyase cDNA ORF Clone, Rat, N-HA tag RG81324-NY
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    Adenylosuccinate Lyase qPCR Primer Pairs, Human, Related Products

    Product Name Catalog#(PDF)
    Adenylosuccinate Lyase cDNA ORF Clone in Cloning Vector, Human HG11287-M
    Adenylosuccinate Lyase cDNA ORF Clone, Human, untagged HG11287-UT
    Adenylosuccinate Lyase Lentiviral cDNA ORF Clone, Human, C-GFPSpark® tag HG11287-ACGLN
    Adenylosuccinate Lyase cDNA ORF Clone, Human, C-GFPSpark® tag HG11287-ACG
    Adenylosuccinate Lyase cDNA ORF Clone, Human, N-GFPSpark® tag HG11287-ANG
    Adenylosuccinate Lyase cDNA ORF Clone, Human, C-OFPSpark® tag HG11287-ACR
    Adenylosuccinate Lyase cDNA ORF Clone, Human, N-OFPSpark® tag HG11287-ANR
    Adenylosuccinate Lyase cDNA ORF Clone, Human, C-DDK (Flag®) tag HG11287-CF
    Adenylosuccinate Lyase cDNA ORF Clone, Human, N-DDK (Flag®) tag HG11287-NF
    Adenylosuccinate Lyase cDNA ORF Clone, Human, C-His tag HG11287-CH
    Adenylosuccinate Lyase cDNA ORF Clone, Human, N-His tag HG11287-NH
    Adenylosuccinate Lyase cDNA ORF Clone, Human, C-HA tag HG11287-CY
    Adenylosuccinate Lyase cDNA ORF Clone, Human, N-HA tag HG11287-NY
    Adenylosuccinate Lyase cDNA ORF Clone, Human, C-Myc tag HG11287-CM
    Adenylosuccinate Lyase cDNA ORF Clone, Human, N-Myc tag HG11287-NM
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