Histone cluster 2 H2BE / HIST2H2BE Protein (Native)

Histone cluster 2, H2be Protein Datasheet

Histone cluster 2 H2BE / HIST2H2BE Protein Product Information

• Synonym: GL105, H2B, H2B.1, H2BFQ, H2BGL105, H2BQ, MGC119802, MGC119804, MGC129733, MGC129734
• Protein Construction: A DNA sequence encoding the human HIST2H2BE (Q16778) (Met 1-Lys 126 ) was expressed and purified.
• Source: Human
• Expression Host: E. Coli

Histone cluster 2 H2BE / HIST2H2BE Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human HIST2H2BE consists of 126 amino acids and has a calculated molecular mass of 14.2 KDa. It migrates as an 16 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 2mM β-Mercaptoethanol, pH 6.9
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: Histone cluster 2 H2BE / HIST2H2BE protein

Histone cluster 2 H2BE / HIST2H2BE Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Histone cluster 2 H2BE / HIST2H2BE Protein Description

Histones are a complex family of highly conserved basic proteins responsible for packaging chromosomal DNA into nucleosomes. There are subtype diversities: H1, H2A, H2B and H3 or H4. It has become more and more evident that histone modifications are key players in the regulation of chromatin states and dynamics as well as in gene expression. Therefore, histone modifications and the enzymatic machineries that set them are crucial regulators that can control cellular proliferation, differentiation, plasticity, and malignancy processes. However, extracellular histones are a double-edged sword because they also damage host tissue and may cause death. Histones bound to platelets, induced calcium influx, and recruited plasma adhesion proteins such as fibrinogen to induce platelet aggregation. Histone H2B proteins have been studied in a variety of species and is easily detecred in most species. The reversible ubiquitylation of histone H2B has long been implicated in transcriptional activation and gene silencing. Phosphorylation of H2B serine 32 occurs in normal cycling and mitogen-stimulated cells. Notably, this phosphorylation is elevated in skin cancer cell lines and tissues compared with normal counterparts.HIST2H2BE is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

References

1. Fuchs TA. et al., 2011, Blood. 118 (13): 3708-14.
2. Collart D. et al., 1993, J Cell Biochem. 50 (4): 374-85.
3. Marzluff WF. et al., 2002, Genomics. 80 (5): 487-98.