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Human HEXA Gene cDNA Clone (full-length ORF Clone)
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Human HEXA Gene cDNA Clone (full-length ORF Clone)

Catalog Vector (Price) Quantity In Stock Operation
HG12037-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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HEXA cDNA Clone Datasheet

 

HEXA cDNA Clone Product Information

Gene Bank Ref.ID: NM_000520

cDNA Size:

1590

Gene Synonym:

HEXA

Source: Human

HEXA cDNA Clone Description

cDNA Description:

ORF Clone of Homo sapiens hexosaminidase A (alpha polypeptide) DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations:1306 A/G resulting in the amino acid Ile substitution by Val and 1518 A/G not causing the amino acid variation.

HEXA cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HEXA cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.

HEXA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACAAGCT CCAGGCTTTG GTTTTCGCTG CTGCTGGCGG CAGCGTTCGC AGGACGGGCG
61       ACGGCCCTCT GGCCCTGGCC TCAGAACTTC CAAACCTCCG ACCAGCGCTA CGTCCTTTAC
121      CCGAACAACT TTCAATTCCA GTACGATGTC AGCTCGGCCG CGCAGCCCGG CTGCTCAGTC
181      CTCGACGAGG CCTTCCAGCG CTATCGTGAC CTGCTTTTCG GTTCCGGGTC TTGGCCCCGT
241      CCTTACCTCA CAGGGAAACG GCATACACTG GAGAAGAATG TGTTGGTTGT CTCTGTAGTC
301      ACACCTGGAT GTAACCAGCT TCCTACTTTG GAGTCAGTGG AGAATTATAC CCTGACCATA
361      AATGATGACC AGTGTTTACT CCTCTCTGAG ACTGTCTGGG GAGCTCTCCG AGGTCTGGAG
421      ACTTTTAGCC AGCTTGTTTG GAAATCTGCT GAGGGCACAT TCTTTATCAA CAAGACTGAG
481      ATTGAGGACT TTCCCCGCTT TCCTCACCGG GGCTTGCTGT TGGATACATC TCGCCATTAC
541      CTGCCACTCT CTAGCATCCT GGACACTCTG GATGTCATGG CGTACAATAA ATTGAACGTG
601      TTCCACTGGC ATCTGGTAGA TGATCCTTCC TTCCCATATG AGAGCTTCAC TTTTCCAGAG
661      CTCATGAGAA AGGGGTCCTA CAACCCTGTC ACCCACATCT ACACAGCACA GGATGTGAAG
721      GAGGTCATTG AATACGCACG GCTCCGGGGT ATCCGTGTGC TTGCAGAGTT TGACACTCCT
781      GGCCACACTT TGTCCTGGGG ACCAGGTATC CCTGGATTAC TGACTCCTTG CTACTCTGGG
841      TCTGAGCCCT CTGGCACCTT TGGACCAGTG AATCCCAGTC TCAATAATAC CTATGAGTTC
901      ATGAGCACAT TCTTCTTAGA AGTCAGCTCT GTCTTCCCAG ATTTTTATCT TCATCTTGGA
961      GGAGATGAGG TTGATTTCAC CTGCTGGAAG TCCAACCCAG AGATCCAGGA CTTTATGAGG
1021     AAGAAAGGCT TCGGTGAGGA CTTCAAGCAG CTGGAGTCCT TCTACATCCA GACGCTGCTG
1081     GACATCGTCT CTTCTTATGG CAAGGGCTAT GTGGTGTGGC AGGAGGTGTT TGATAATAAA
1141     GTAAAGATTC AGCCAGACAC AATCATACAG GTGTGGCGAG AGGATATTCC AGTGAACTAT
1201     ATGAAGGAGC TGGAACTGGT CACCAAGGCC GGCTTCCGGG CCCTTCTCTC TGCCCCCTGG
1261     TACCTGAACC GTATATCCTA TGGCCCTGAC TGGAAGGATT TCTACGTAGT GGAACCCCTG
1321     GCATTTGAAG GTACCCCTGA GCAGAAGGCT CTGGTGATTG GTGGAGAGGC TTGTATGTGG
1381     GGAGAATATG TGGACAACAC AAACCTGGTC CCCAGGCTCT GGCCCAGAGC AGGGGCTGTT
1441     GCCGAAAGGC TGTGGAGCAA CAAGTTGACA TCTGACCTGA CATTTGCCTA TGAACGTTTG
1501     TCACACTTCC GCTGTGAGTT GCTGAGGCGA GGTGTCCAGG CCCAACCCCT CAATGTAGGC
1561     TTCTGTGAGC AGGAGTTTGA ACAGACCTGA

 

Quality Control:cDNA sequencing results is available upon request.

 

 

Hexosaminidase A (HEXA) related areas, pathways, and other information

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"