HPX / Hemopexin Antibody (Antigen Affinity Purified)

Hemopexin / HPX Antibody ( Antigen Affinity Purified )

Hemopexin / HPX Antibody Product Information

• Immunogen: Recombinant human HPX protein ( Catalog#10870-H08H )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human HPX / Hemopexin ( rh HPX; Catalog#10870-H08H; Met 1- His 462; NP_000604.1 ). HPX specific IgG was purified by human HPX affinity chromatography.

Hemopexin / HPX Antibody Usage Guide

• Specificity: Human HPX / Hemopexin
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect in WB. Using a DAB detection system, the detection limit for Human HPX is approximately 2 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human HPX. The detection limit for human HPX is 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Hemopexin / HPX Antibody Background

Human Hemopexin ( HPX ) is a serum glycoprotein responsible for the transport of haem to tissues such as liver, by receptor-mediated endocytosis, in an analogous manner to the iron transport protein transferrin, with recycling of intact haemopexin. Hemopexin binds heme with the highest affinity of any known protein. Its performs functions in scavenging the heme released or lost by the turnover of heme proteins such as hemoglobin, protects the body from the oxidative damage that free heme can cause. In addition, hemopexin releases its bound ligand for internalisation upon interacting with a specific receptor situated on the surface of liver cells, thus implicated in preservation of the body's iron. High hemopexin levels indicate that there has been significant degradation of heme containing compounds and hemopexin is made to scavenge any heme it can, and high hemopexin levels are therefore one of the diagnostic features of a hemolytic anemia.

References

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4. Tolosano, E. et al., 2002,  DNA Cell Biol. 21 (4): 297-306.