HPX / Hemopexin Antibody

Hemopexin / HPX Antibody Datasheet

Hemopexin / HPX Antibody Product Information

• Immunogen: Recombinant human HPX protein ( Catalog#10870-H08H )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human HPX / Hemopexin ( rh HPX; Catalog#10870-H08H; Met 1- His 462; NP_000604.1 ). Total IgG was purified by Protein A affinity chromatography.

Hemopexin / HPX Antibody Usage Guide

• Specificity: Human HPX / Hemopexin
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect in WB.
• Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human HPX. The detection limit for human HPX is 0.0049 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

HPX Antibody Related Products &Topics

Related Areas:

Immunology>>Innate Immunity>>Acute Phase Proteins>>Hemopexin/HPX

Proteins:

Molecule	Species	Description	Cat. No
Hemopexin/HPX	Human	Hemopexin/HPX Protein, Recombinant	10870-H08H
Hemopexin/HPX	Mosue	Hemopexin/HPX Protein, Recombinant	50271-M08H

Antibodies:

Molecule	Application	Description	Cat. No
Human Hemopexin/HPX	WB, ELISA	Mouse Monoclonal Antibody	10870-MM01
Human Hemopexin/HPX	ELISA	Mouse Monoclonal Antibody	10870-MM02
Human Hemopexin/HPX	WB, ELISA	Rabbit Polyclonal Antibody	10870-RP03
Human Hemopexin/HPX	WB, ELISA	Rabbit Polyclonal Antibody (Antigen Affinity Purified)	10870-RP04
Mouse Hemopexin/HPX	WB, ELISA	Hemopexin/HPX Antibody, Rabbit PAb	50271-RP01
Mouse Hemopexin/HPX	WB, ELISA	Hemopexin/HPX Antibody, Rabbit PAb (Antigen Affinity Purified)	50271-RP02

Hemopexin / HPX Antibody Background

Human Hemopexin ( HPX ) is a serum glycoprotein responsible for the transport of haem to tissues such as liver, by receptor-mediated endocytosis, in an analogous manner to the iron transport protein transferrin, with recycling of intact haemopexin. Hemopexin binds heme with the highest affinity of any known protein. Its performs functions in scavenging the heme released or lost by the turnover of heme proteins such as hemoglobin, protects the body from the oxidative damage that free heme can cause. In addition, hemopexin releases its bound ligand for internalisation upon interacting with a specific receptor situated on the surface of liver cells, thus implicated in preservation of the body's iron. High hemopexin levels indicate that there has been significant degradation of heme containing compounds and hemopexin is made to scavenge any heme it can, and high hemopexin levels are therefore one of the diagnostic features of a hemolytic anemia.

References

1. Takahashi, N. et al.,1984,  Proc. Natl. Acad. Sci. USA. 81 (7): 2021-5.
2. Smith, A. et al.,1993,  J. Biol. Chem. 268 (10): 7365-71.
3. Morris, CM. et al.,1993,  Neurosci. Lett. 149 (2): 141-4.
4. Tolosano, E. et al., 2002,  DNA Cell Biol. 21 (4): 297-306.