Mouse HVEM / TNFRSF14 ORF Expression Clone, untagged (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
MG50550-M-N	pCMV / hygro ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

HVEM / TNFRSF14 cDNA Clone Datasheet

HVEM / TNFRSF14 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_178931.2
cDNA Size:	828
Gene Synonym:	Atar; HveA; Hvem; Tnfrs14; MGC123498; MGC123499; Tnfrsf14
Source:	Mouse

HVEM / TNFRSF14 cDNA Clone Description

cDNA Description:	ORF Clone of Mus musculus tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) DNA.
Vector:	pCMV/hygro
Sequence Description:	Identical with the Gene Bank Ref. ID sequence.

HVEM / TNFRSF14 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HVEM / TNFRSF14 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HVEM / TNFRSF14 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAACCTC TCCCAGGATG GGGGTCGGCA CCCTGGAGCC AGGCCCCTAC AGACAACACC
61       TTCAGGCTGG TGCCTTGTGT CTTCCTTTTG AACTTGCTGC AGCGCATCTC TGCCCAGCCC
121      TCATGCAGAC AGGAGGAGTT CCTTGTGGGA GACGAGTGCT GCCCCATGTG CAACCCAGGT
181      TACCATGTGA AGCAGGTCTG CAGTGAGCAT ACAGGCACAG TGTGTGCCCC CTGTCCCCCA
241      CAGACATATA CCGCCCATGC AAATGGCCTG AGCAAGTGTC TGCCCTGCGG AGTCTGTGAT
301      CCAGACATGG GCCTGCTGAC CTGGCAGGAG TGCTCCAGCT GGAAGGACAC TGTGTGCAGA
361      TGCATCCCAG GCTACTTCTG TGAGAACCAG GATGGGAGCC ACTGTTCCAC ATGCTTGCAG
421      CACACCACCT GCCCTCCAGG GCAGAGGGTA GAGAAGAGAG GGACTCACGA CCAGGACACT
481      GTATGTGCTG ACTGCCTAAC AGGGACCTTC TCACTTGGAG GGACTCAGGA GGAATGCCTG
541      CCCTGGACCA ACTGCAGTGC ATTTCAACAG GAAGTAAGAC GTGGGACCAA CAGCACAGAC
601      ACCACCTGCT CCTCCCAGGT CGTCTACTAC GTTGTGTCCA TCCTTTTGCC ACTTGTGATA
661      GTGGGAGCTG GGATAGCTGG ATTCCTCATC TGCACGCGAA GACACCTGCA CACCAGCTCA
721      GTGGCCAAGG AGCTGGAGCC TTTCCAGGAA CAACAGGAGA ACACCATCAG GTTTCCAGTC
781      ACCGAGGTTG GGTTTGCTGA GACCGAGGAG GAGACAGCCT CCAACTAA

Quality Control:cDNA sequencing results is available upon request.

Cytokine