Mouse HTR4 Gene cDNA Clone / ORF Clone

HTR4 cDNA Clone Datasheet

HTR4 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_008313.4
• cDNA Size: 1167
• Gene Synonym: 5-HT4; 5-HT<4L>; Htr4
• Source: Mouse

HTR4 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus 5 hydroxytryptamine (serotonin) receptor 4 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 309 T/C not causing the amino acid variation.

HTR4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HTR4 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HTR4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACAAAC TTGATGCTAA TGTGAGTTCC AACGAGGGTT TCAGGTCCGT GGAGAAGGTC
61       GTGCTGCTCA CGTTCCTTGC AGTGGTTATC CTGATGGCCA TCTTGGGCAA CCTGCTGGTG
121      ATGGTGGCTG TGTGCAGGGA CAGGCAGCTC AGGAAAATAA AAACCAACTA TTTCATTGTG
181      TCTCTCGCCT TTGCTGACCT GCTGGTTTCG GTGCTGGTGA TGCCCTTTGG TGCCATTGAG
241      CTGGTCCAAG ACATCTGGGC TTATGGGGAG ATGTTCTGCC TGGTCCGGAC CTCTCTGGAT
301      GTCCTACTCA CCACAGCATC GATCTTTCAC CTGTGCTGTA TTTCCCTGGA CAGGTATTAC
361      GCCATCTGCT GCCAGCCTTT GGTTTATAGG AACAAGATGA CCCCTCTACG CATCGCATTA
421      ATGTTGGGAG GCTGCTGGGT CCTTCCCATG TTTATATCTT TTCTCCCCAT AATGCAAGGC
481      TGGAACAACA TCGGCATAGT TGATGTGATA GAGAAAAGGA AATTCAGCCA CAACTCTAAC
541      TCCACGTGGT GTGTCTTCAT GGTCAACAAG CCCTATGCTA TCACCTGCTC TGTGGTGGCC
601      TTCTACATCC CGTTTCTCCT CATGGTGCTG GCCTATTACC GAATCTATGT CACTGCTAAG
661      GAGCATGCCC AGCAGATACA GATGTTACAA CGGGCAGGAG CCACCTCTGA AAGCAGGCCC
721      CAGCCAGCTG ACCAGCACAG CACACATCGC ATGAGGACAG AGACCAAGGC AGCCAAGACT
781      TTATGTGTCA TCATGGGCTG CTTCTGTTTC TGCTGGGCCC CCTTCTTTGT CACCAATATT
841      GTGGACCCTT TCATAGACTA CACTGTCCCC GAGCAGGTGT GGACTGCTTT CCTCTGGCTT
901      GGCTATATCA ATTCGGGGTT GAACCCTTTT CTCTATGCCT TCTTGAATAA GTCTTTCAGA
961      CGTGCCTTCC TCATCATCCT CTGCTGTGAT GATGAGCGCT ACAAAAGACC CCCCATTCTG
1021     GGCCAGACTG TCCCCTGTTC AACCACAACC ATTAATGGAT CCACCCATGT ACTAAGGGAT
1081     ACAGTGGAAT GTGGTGGCCA GTGGGAGAGT CGGTGTCACC TCACAGCAAC TTCTCCTTTG
1141     GTGGCTGCTC AGCCCAGTGA TACTTAA

 

Quality Control:cDNA sequencing results is available upon request.