HSPD1 / HSP60 Antibody (Antigen Affinity Purified)

HSPD1 / HSP60 Antibody ( Antigen Affinity Purified ) Datasheet

HSPD1 / HSP60 Antibody Product Information

• Immunogen: Recombinant Mouse HSPD1 / HSP60 protein (Catalog#50596-M07E)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse HSPD1 / HSP60 (rM HSPD1 / HSP60; Catalog#50596-M07E; NP_034607.3; Leu 2-Phe 573). HSPD1 / HSP60 specific IgG was purified by Mouse HSPD1 / HSP60 affinity chromatography.

HSPD1 / HSP60 Antibody Usage Guide

• Specificity: Mouse HSPD1 / HSP60
• Immunochemistry: IHC-P: 0.05-2 μg/mL

  

  Immunochemical staining of mouse HSP60 in mouse kidney with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to nephric tubule.

  Immunochemical staining of mouse HSP60 in mouse intestine with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).

  

  Immunochemical staining of mouse HSP60 in mouse lung with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).

• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse HSP60 in WB. Using a DAB detection system, the detection limit for Mouse HSP60 is approximately 2 ng/lane under non-reducing conditions and 16 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse HSP60. The detection limit for Mouse HSP60 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

HSPD1 / HSP60 Antibody Background

60 kDa heat shock protein, mitochondrial, also known as 60 kDa chaperonin, Chaperonin 60, Heat shock protein 60, HSP-60, Mitochondrial matrix protein P1, HSPD1 and HSP60, is a mitochondrion matrix protein which belongs to thechaperonin (HSP60) family. HSPD1/HSP60 is implicated in mitochondrial protein import and macromolecular assembly. It may facilitate the correct folding of imported proteins. HSPD1/HSP60 may also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. Defects in HSPD1/HSP60 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) which is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Defects in HSPD1/HSP60 are also the cause of leukodystrophy hypomyelinating type 4 which is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life.

References

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2. Rasmussen R.K., et al., 1997, Electrophoresis 18:588-98.
3. Hansen J.J., et al., 2002, Am. J. Hum. Genet. 70:1328-32.
4. Hansen J.J., et al., 2003, Hum. Genet. 112:71-77.
5. Magen D., et al., 2008, Am. J. Hum. Genet. 83:30-42.