Mouse HSPB8 Gene cDNA Clone / ORF Clone

HSPB8 cDNA Clone Datasheet

HSPB8 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_030704.2
• cDNA Size: 591
• Gene Synonym: H11; H11K; Cryac; E2IG1; HSP22; D5Ucla4; AU018630; AW413033; HSP20-like; Hspb8
• Source: Mouse

HSPB8 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus heat shock protein 8 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

HSPB8 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

HSPB8 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

HSPB8 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGACG GGCAATTGCC TTTCCCGTGC TCCTACCCAA GCCGCCTGCG CCGAGACCCC
61       TTTCGGGACT CACCGCTCTC CTCCCGCCTT CTGGACGATG GCTTTGGCAT GGACCCTTTT
121      CCAGACGACT TGACAGCCCC TTGGCCAGAG TGGGCTTTGC CTCGGCTCTC CTCTGCCTGG
181      CCTGGAACCC TAAGGTCTGG CATGGTACCC CGAGGGCCTC CAGCCACAGC TAGGTTTGGA
241      GTGCCCGCTG AGGGCAGGAG TCCCCCACCC TTCCCTGGGG AGCCTTGGAA AGTGTGTGTC
301      AACGTGCACA GCTTCAAGCC GGAAGAGCTG ATGGTAAAGA CCAAGGATGG ATACGTGGAA
361      GTTTCAGGCA AACATGAAGA GAAGCAGCAG GAAGGTGGGA TTGTCTCCAA GAACTTCACC
421      AAGAAAATAC AGCTCCCTGC AGAAGTGGAT CCAGCCACTG TATTTGCCTC GCTTTCTCCA
481      GAGGGCCTGC TCATCATCGA GGCTCCCCAG GTCCCTCCGT ACTCACCCTT TGGAGAGAGC
541      AGCTTCAACA ACGAGCTTCC TCAAGACAAC CAGGAAGTCA CCTGTTCCTA A

 

Quality Control:cDNA sequencing results is available upon request.