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HSP70 / HSPA1A Antibody, Rabbit MAb

  • HSP70 / HSPA1A Antibody, Rabbit MAb, Immunochemistry
  • HSP70 / HSPA1A Antibody, Rabbit MAb, Immunochemistry
  • Human HSP70/HSPA1A Western blot (WB) 14774
  • Human HSP70/HSPA1A Immunoprecipitation(IP) 14812
  • HSP70 / HSPA1A Antibody, Rabbit MAb, Flow Cytometry
  • HSP70 / HSPA1A Antibody, Rabbit MAb, Immunofluorescence
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Human HSPA1A Antibody Product Information
Immunogen:Recombinant Human HSP70 / HSPA1A protein (Catalog#11660-H07B)
Clone ID:001
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human HSP70 / HSPA1A (rh HSP70 / HSPA1A; Catalog#11660-H07B; P08107; Ala2-Asp641).
Human HSPA1A Antibody IHC Application Image
HSP70 / HSPA1A Antibody, Rabbit MAb, Immunochemistry
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Caption:
Immunochemical staining of HSPA1A in cynomolgus macaque testis with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
HSP70 / HSPA1A Antibody, Rabbit MAb, Immunochemistry
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Caption:
Immunochemical staining of HSPA1A in human hepatoma with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
Human HSPA1A Antibody IF Application Image
HSP70 / HSPA1A Antibody, Rabbit MAb, Immunofluorescence
[Click to enlarge image]
Caption:
Immunofluorescence staining of Human HSPA1A in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human HSPA1A monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
Human HSPA1A Antibody FC Application Image
HSP70 / HSPA1A Antibody, Rabbit MAb, Flow Cytometry
[Click to enlarge image]
Caption:
Flow cytometric analysis of Human HSPA1A expression on HeLa cells . The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human HSPA1A, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Other HSPA1A Antibody Products
Immunochemical staining of human CCNF in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
HSP70/HSPA1A Background

HSPA1A is a member of the Hsp70 protein family. The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. HSP are abundant and conserved proteins present in all cells. Upon temperature shock or other stress stimuli, HSP are synthesized intracellularly, which may protect cells from protein denaturation or from death. Extracellularly, HSP can serve a cytokine function to initiate both innate and adaptive immunity through activation of APC. HSP serves also a chaperone function and facilitates presentation of antigen peptide to T cells. Molecular chaperones of the Hsp70 family have diverse functions in cells. They assist the folding of newly synthesized and stress-denatured proteins, as well as the import of proteins into organelles, and the dissociation of aggregated proteins. The well-conserved Hsp70 chaperones are ATP dependent: binding and hydrolysis of ATP regulates their interactions with unfolded polypeptide substrates, and ATPase cycling is necessary for their function. All cellular functions of Hsp70 chaperones use the same mechanism of ATP-driven polypeptide binding and release. 

Human HSP70/HSPA1A References
  • Heck TG, et al. (2011) HSP70 expression: does it a novel fatigue signalling factor from immune system to the brain Cell Biochem Funct. 29 (3): 215-26.
  • Chen T, et al. (2010) Stress for maintaining memory: HSP70 as a mobile messenger for innate and adaptive immunity. Eur J Immunol. 40 (6): 1541-4.
  • Young JC. (2010) Mechanisms of the Hsp70 chaperone system. Biochem Cell Biol. 88 (2): 291-300.
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    Catalog: 11660-R001-50
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    Datasheet & Documentation

    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"